MitoPediaAlert2020 Archive

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

June 2020

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• Jun-04 Th

Does your sample display a preference for glutamate & malate over pyruvate & malate to support NADH-linked respiration? DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates. -»SUIT-008« and »SUIT-014«

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Previous MitoPedia Alert 2020

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May 2020

• May-28 Th

Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? - »Nitrogen injection«

• May-21 Th

As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production. -»PhotoBiology«

• May-14 Th

How can I measure ATP production with the O2k? Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green - »Magnesium Green«

• May-07 Th

Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it? Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present? - Learn more about - »malate anaplerotic pathway control state«  - DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«  - Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«

Apr 2020

• Apr-24 Fr

ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system. - »Hatefi 1962 J Biol Chem-XLII«

• Apr-21 Tu

Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature. - »MiPNet17.04 CitrateSynthase«

• Apr-13 Mo

Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases? DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation - »SUIT-024«

• Apr-08 We

The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage. DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration. - »SUIT-010«

• Apr-02 Th

Do you want to search the best SUIT protocol for your research questions? - Use the SUITbrowser: »SUITbrowser«

Mar 2020

• Mar-16 Mo

The standardized coupling control protocol to explore the bioenergetics of living cells DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«

• Mar-11 We

Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - »Blebbistatin«

• Mar-06 Fr

Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols. - »RP1« and »RP2«

• Mar-03 Tu

Rapamycin - more differences or more in common with other mitObesity drugs? - »Rapamycin«

Feb 2020

• Feb-26 We

It is essential to titrate uncouplers in multiple steps for respirometric measurement of ET capacity. - »Noncoupled respiration«

• Feb-24 Mo

More research is needed on the impact of viral infection on mitochondrial function. - »Viruses and mitochondrial medicine«

• Feb-19 We

Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - »Spermidine«

• Feb-18 Tu

Why do we fight gas bubbles? - Dive deep into »Oxygen solubility«

• Feb-12 We

What do metformin, melatonin and other bioactive mitObesity compounds have in common? - »MitObesity drugs«

Jan 2020

• Jan-16 Th

Oxygen flux - which SI units should be used?

Did you ever think that units for body mass could be an issue? - »Body mass«

• Jan-08 We

Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.