NextGen-O2k Documentation

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP3: Documentation

Abstract

In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.

Achievements

Project Year 1: In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.

Project Year 2: Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module. These materials provide step-by-step instructions, support and information for using the NextGen-O2k. Furthermore, trailers for the new modules (Q, NADH and PB) were recorded and published.

Progress and next steps

See » Track record

NextGen-O2k Videosupport clips

Q-Module (NextGen-O2k - DatLab 7)

• Q-service

Assembly of the reference electrode
NG Polishing glassy carbon and platinum electrodes
NG O2k-Chamber volume calibration with Q-Sensor

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• Q-experiments

NG Q-experiments

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• Cleaning Q-Sensors

NG Cleaning after cyclic voltammetry
NG Cleaning after Q-experiments

FluoRespirometry

Smart Fluo-Sensor assembly
Exchanging a filter set of the smart-fluo sensor
H₂O₂ flux analysis with DatLab 7.4

O2k-Mitochondrial preparations

Shredder tissue homogenate
Fiber permeabilization
Addition of biological sample to the respirometer chamber

Quality Control

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration

NextGen-O2k Manuals

» NextGen-O2k manual

» DatLab8.0: CV-Module

» NextGen-O2k: Q-Module

» NextGen-O2k: NADH-Module

» NextGen-O2k: PB-Module

» O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8

» Oxygen flux_analysis - DatLab 7.4

» Safranin Analysis Template

» General template for mt-membrane potential analysis

» H₂O₂ flux analysis