O2k-Open Support alert: Difference between revisions
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::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background can be performed in the O2k-sV-Module. - Β»'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''Β« | ::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background can be performed in the O2k-sV-Module. - Β»'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''Β« | ||
* '''Mar-11 We''' | * '''Mar-11 We''' |
Revision as of 14:58, 19 March 2020
O2k-Open Support alert |
- Currently Answered Questions, CAQ, from the Β»Oroboros Ecosystem AgendaΒ«
Mar 2020
- Mar-23 We
- New Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
- (1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background can be performed with the "BG Feedback" settings. - Β»O2k-sV-ModuleΒ«
- (2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module, the instrumental O2 background can be performed in the O2k-sV-Module. - Β»O2k-sV-ModuleΒ«
- New Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
- Mar-11 We
- New Do you change the slope smoothing to 20 while running experiments with biological sample?
- While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - Β»Slope smoothingΒ«
- New Do you change the slope smoothing to 20 while running experiments with biological sample?
- Mar-04 We
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - Β»O2k-OroboPOS-HolderΒ«
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
Feb 2020
- Feb-28 Fr
- New Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - Β» Oxygen calibration - DatLabΒ«
- New Calibration parameters are transferred to experimental DatLab files
Jan 2020
- Jan-31 Fr
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - Β»Oxygen calibration - DatLabΒ«
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- Jan-30 Th
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - Β»Oxygen calibration - DatLabΒ«
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- Your air calibration is not effective until you set mark βR1β and click on Calibrate and copy to clipboard in the O2 calibration window. - Β»Oxygen calibration - DatLabΒ«
- Jan-24 Fr
- Do you write the serial number of the polarographic oxygen sensor (POS) in the βO2k configurationβ window?
- Including the POS serial number in the Β΄O2k configurationΒ΄ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. Β»O2k configurationΒ«
- Do you write the serial number of the polarographic oxygen sensor (POS) in the βO2k configurationβ window?
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - Β»O2k-Chamber MitoPediaΒ«
- Do you perform O2k-Chamber volume calibration?
- Do you use the correct O2 solubility factor (FM) in the βO2 calibrationβ window in DatLab?
- The O2 solubility factor varies according to the medium used and influences the air calibration. - Β»Oxygen solubility factorΒ«
- Do you use the correct O2 solubility factor (FM) in the βO2 calibrationβ window in DatLab?
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