Difference between revisions of "Onopiuk 2009 Biochem Biophys Res Commun"
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}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Genetic knockout;overexpression | |||
|organism=Mouse | |organism=Mouse | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Intact cells, Permeabilized cells | |preparations=Intact cells, Permeabilized cells | ||
|injuries=RONS; Oxidative Stress | |injuries=RONS; Oxidative Stress | ||
|diseases=Muscular dystrophy | |||
|topics=ATP; ADP; AMP; PCr, Ion&substrate transport | |topics=ATP; ADP; AMP; PCr, Ion&substrate transport | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS |
Revision as of 13:23, 12 August 2013
Onopiuk M, Brutkowski W, Wierzbicka K, Wojciechowska S, Szczepanowska J, Fronk J, Lochmüller H, Górecki DC, Zablocki K (2009) Mutation in dystrophin-encoding gene affects energy metabolism in mouse myoblasts. Biochem Biophys Res Commun 386: 463-466. |
Onopiuk M, Brutkowski W, Wierzbicka K, Wojciechowska S, Szczepanowska J, Fronk J, Lochmueller H, Gorecki DC, Zablocki K (2009) Biochem Biophys Res Commun
Abstract: Duchenne Muscular Dystrophy is characterized by severe defects in differentiated muscle fibers, including abnormal calcium homeostasis and impaired cellular energy metabolism. Here we demonstrate that myoblasts derived from dystrophic (mdx) mouse exhibit reduced oxygen consumption, increased mitochondrial membrane potential, enhanced reactive oxygen species formation, stimulated glycolysis but unaffected total cellular ATP content. Moreover, reduced amounts of specific subunits of the mitochondrial respiratory complexes and ATP-synthase as well as disorganized mitochondrial network were observed. Both the dystrophic and control myoblasts used were derived from a common inbred mouse strain and the only difference between them is a point mutation in the dystrophin-encoding gene, thus these data indicate that this mutation results in multiple phenotypic alterations demonstrating as early as in undifferentiated myoblasts. This finding sheds new light on the molecular mechanisms of Duchenne Muscular Dystrophy pathogenesis. • Keywords: mdx myoblast, Mitochondrial potential, Reactive oxygen species, Respiration, Mitochondrial organization
• O2k-Network Lab: PL Warsaw Zablocki K
Labels: MiParea: Respiration, Genetic knockout;overexpression
Pathology: Muscular dystrophy"Muscular dystrophy" is not in the list (Aging;senescence, Alzheimer's, Autism, Cancer, Cardiovascular, COPD, Diabetes, Inherited, Infectious, Myopathy, ...) of allowed values for the "Diseases" property.
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.
Organism: Mouse
Tissue;cell: Skeletal muscle
Preparation: Intact cells, Permeabilized cells
Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Ion&substrate transport"Ion&substrate transport" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
HRR: Oxygraph-2k