Oroboros Laboratory
About us
The OROBOROS MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project MitoCom Tyrol, June 2013. It comprises 140 sqm which are divided into the office and research areas.
O2k instruments
28 O2k-Chambers (14 O2k)
- Twelve O2k instruments (24 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.
- Two additional O2ks (4 chambers) are devoted to instrumental development.
More details: OROBOROS Support
OXPHOS analysis
SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
- a phosphorylation control protocol in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
- various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry).
The MitoFit Centre of Excellence
OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.
According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.
Current projects and collaborators
- The OROBOROS-Team collaborates with:
Pharmacology: mitochondrial function in brain tissues
- Johannes Burtscher, Mag. PhD
- FWF projects W1206-B05 and I-977-B24.
- Project leader: A.Univ. Prof. Dr. Christian Schwarzer, Dept. Pharmacology, Medical University Innsbruck, Austria
Internal Medicine
- Chiara Volani, [email protected]
- Project leader: Prof. Dr. GΓΌnther Weiss, Dept. of Internal Medicine, Medical University Innsbruck, Austria
MitoFit project
- Florian Hoppel, MSc.
- PhD student (discontinued)
- Institute for Sports Science and OROBOROS: K-Regio MitoFit
Oncotyrol
- Prostate cancer
- The Oncotyrol research project has its focus on functional differences of mitochondria in human prostate tumor versus benign tissue, and comparison of primary tissue with cultured cells. - Oncotyrol
- Project leader: A.Univ. Prof. Mag. Dr. Helmut Klocker, Dept. Urology, Medical University Innsbruck, Austria
- Anja Weber, Mag., PhD student - [email protected]
- Bernd Schoepf, Mag., PhD student -