Ouabain: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Oua | |abbr=Oua | ||
|description=Ouabain (G-strophantin octahydrate | |description='''Ouabain''' (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. At low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase. | ||
|info=[[MiPNet03.02]] | |info=[[MiPNet03.02]] | ||
}} {{MitoPedia topics | }} {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
== Application in [[HRR]] == | |||
'''Oua: Ouabain octahydrate''' (≥95% (HPLC), powder; C<sub>29</sub>H<sub>44</sub>O<sub>12</sub><sub>*</sub>8H<sub>2</sub>0); Sigma O 3125, store at R.T.; FW = 728.77 | |||
<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!.</span> | |||
'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | |||
::1) weigh 7.3 mg of Ouabain | |||
::2) dissolve in 1 ml H<sub>2</sub>O | |||
::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution | |||
::4) store frozen in glass vials (recommendation) at -20 °C | |||
Revision as of 10:06, 18 May 2012
- high-resolution terminology - matching measurements at high-resolution
Ouabain
Description
Ouabain (synonym: G-strophantin octahydrate) is a poisonous cardiac glycoside. The classical mechanism of action of ouabain involves its binding to and inhibition of the plasma membrane Na+/K+-ATPase (sodium pump) especially at the higher concentrations. At low (nanomolar and subnanomolar) concentrations of ouabain stimulate the Na-K-ATPase.
Abbreviation: Oua
Reference: MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Oua: Ouabain octahydrate (≥95% (HPLC), powder; C29H44O12*8H20); Sigma O 3125, store at R.T.; FW = 728.77
Caution: Light sensitive and toxic!.
Preparation of 10 mM stock solution (dissolved in H2O):
- 1) weigh 7.3 mg of Ouabain
- 2) dissolve in 1 ml H2O
- 3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 4) store frozen in glass vials (recommendation) at -20 °C
