Palmitoyl-CoA: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Pa-CoA: Palmitoyl-CoA''' (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20ยฐC; FW=1004.94) | :::'''Pa-CoA: Palmitoyl-CoA''' (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20ยฐC; FW=1004.94) | ||
'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | ||
::::# Weigh 5 mg Palmitoyl-CoA. | ::::# Weigh 5 mg Palmitoyl-CoA. | ||
::::# Add 497.5 ยตL H<sub>2</sub>O. | ::::# Add 497.5 ยตL H<sub>2</sub>O. | ||
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::::# Store at -20ยฐC. | ::::# Store at -20ยฐC. | ||
'''O2k manual titrations''' | :::ยป'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 8 ยตL 10 mM using a 25 ยตL Hamilton syringe (2 mL O2k-Chamber). | ::::* Titration volume: 8 ยตL 10 mM using a 25 ยตL Hamilton syringe (2 mL O2k-Chamber). | ||
::::* Final concentration: 40 ยตM. | ::::* Final concentration: 40 ยตM. | ||
::::* Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration. | ::::* Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration. | ||
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Revision as of 09:42, 11 September 2020
- high-resolution terminology - matching measurements at high-resolution
Palmitoyl-CoA
Description
Palmitoyl-CoA is a coenzyme A derivative of palmitate formed by acyl-CoA synthase. In contrast to medium- and short-chain acyl-CoA, palmitoyl-CoA cannot freely diffuse into the mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior to transfer into mitochondria for further fatty acid oxidation (ฮฒ-oxidation). To study Fatty acid oxidation using Palmitoyl-CoA, Carnitine and low amount of malate is needed on mitochondrial preparations.
Application in HRR
- Pa-CoA: Palmitoyl-CoA (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20ยฐC; FW=1004.94)
- Preparation of 10 mM stock solution (dissolved in H2O):
- Weigh 5 mg Palmitoyl-CoA.
- Add 497.5 ยตL H2O.
- Divide into 100 ยตL portions.
- Store at -20ยฐC.
- Preparation of 10 mM stock solution (dissolved in H2O):
- Pa-CoA: Palmitoyl-CoA (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20ยฐC; FW=1004.94)
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 8 ยตL 10 mM using a 25 ยตL Hamilton syringe (2 mL O2k-Chamber).
- Final concentration: 40 ยตM.
- Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration.
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
MitoPedia topics: Substrate and metabolite
