Palmitoyl-CoA: Difference between revisions

Revision as of 12:03, 15 June 2021

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Palmitoyl-CoA

Description

Palmitoyl-CoA is a coenzyme A derivative of palmitate formed by acyl-CoA synthase. In contrast to medium- and short-chain acyl-CoA, palmitoyl-CoA cannot freely diffuse into the mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior to transfer into mitochondria for further fatty acid oxidation (β-oxidation). To study Fatty acid oxidation using Palmitoyl-CoA, Carnitine and low amount of malate is needed on mitochondrial preparations.

Application in HRR

Pa-CoA: Palmitoyl-CoA (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20°C; M = 1004.94 g·mol^-1

Preparation of 10 mM stock solution (dissolved in H₂O):

Weigh 5 mg Palmitoyl-CoA.
Add 497.5 µL H₂O.
Divide into 100 µL portions.
Store at -20°C.

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume: 8 µL 10 mM using a 25 µL Hamilton syringe (2 mL O2k-Chamber).
Final concentration: 40 µM.
Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration.

MitoPedia topics: Substrate and metabolite

@@ Line 7: / Line 7: @@
 == Application in [[HRR]] ==
-:::'''Pa-CoA: Palmitoyl-CoA''' (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20°C; M = 1004.94 g·mol<sup>-1</sup>)
+:::'''Pa-CoA: Palmitoyl-CoA''' (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20°C; M = 1004.94 g·mol<sup>-1</sup>
 ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
 ::::# Weigh 5 mg Palmitoyl-CoA.