Perna 2016 Amino Acids
Perna MK, Kokenge AN, Miles KN, Udobi KC, Clark JF, Pyne-Geithman GJ, Khuchua Z, Skelton MR (2016) Creatine transporter deficiency leads to increased whole body and cellular metabolism. Amino Acids 48:2057-65. |
Perna MK, Kokenge AN, Miles KN, Udobi KC, Clark JF, Pyne-Geithman GJ, Khuchua Z, Skelton MR (2016) Amino Acids
Abstract: Creatine (Cr) is a guanidino compound required for rapid replenishment of ATP in cells with a high-energy demand. In humans, mutations in the Cr transporter (CRT;SLC6A8) prevent Cr entry into tissue and result in a significant intellectual impairment, epilepsy, and aphasia. The lack of Cr on both the whole body and cellular metabolism was evaluated in Crt knockout (Crt -/y) mice, a high-fidelity model of human CRT deficiency. Crt -/y mice have reduced body mass and, however, show a twofold increase in body fat. There was increased energy expenditure in a home cage environment and during treadmill running in Crt -/y mice. Consistent with the increases in the whole-body metabolic function, Crt -/y mice show increased cellular metabolism as well. Mitochondrial respiration increased in skeletal muscle fibers and hippocampal lysates from Crt -/y mice. In addition, Crt -/y mice had increased citrate synthase activity, suggesting a higher number of mitochondria instead of an increase in mitochondrial activity. To determine if the increase in respiration was due to increased mitochondrial numbers, we measured oxygen consumption in an equal number of mitochondria from Crt +/y and Crt -/y mice. There were no changes in mitochondrial respiration when normalized to mitochondrial number, suggesting that the increase in respiration observed could be to higher mitochondrial content in Crt -/y mice. β’ Keywords: Creatine, Creatine transporter, Creatine transporter deficiency, Mitochondria β’ Bioblast editor: Kandolf G β’ O2k-Network Lab: US OH Cincinnati Clark JF
Labels: MiParea: Respiration, Genetic knockout;overexpression
Organism: Mouse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, NS, ROX
HRR: Oxygraph-2k
Labels, 2017-12