Pinho 2020 PLoS Negl Trop Dis: Difference between revisions
No edit summary |
No edit summary ย |
||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Pinho N, Wiลniewski JR, Dias-Lopes G, Saboia-Vahia L, Bombaรงa ACS, Mesquita-Rodrigues C, Menna-Barreto R, Cupolillo E, de Jesus JB, Padrรณn G, Cuervo P (2020) In-depth quantitative proteomics uncovers specie-specific metabolic programs in ''Leishmania'' (''Viannia'') species. PLoS Negl Trop Dis | |title=Pinho N, Wiลniewski JR, Dias-Lopes G, Saboia-Vahia L, Bombaรงa ACS, Mesquita-Rodrigues C, Menna-Barreto R, Cupolillo E, de Jesus JB, Padrรณn G, Cuervo P (2020) In-depth quantitative proteomics uncovers specie-specific metabolic programs in ''Leishmania'' (''Viannia'') species. PLoS Negl Trop Dis 14:e0008509. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/32804927 PMID: 32804927 Open Access] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/32804927 PMID: 32804927 Open Access] | ||
|authors=Pinho Nathalia, Wisniewski Jacek R, Dias-Lopes Geovane, Saboia-Vahia Leonardo, Souza Bombaca Ana Cristina, Mesquita-Rodrigues Camila, Menna-Barreto Rubem, Cupolillo Elisa, de Jesus Jose Batista, Padron Gabriel, Cuervo Patricia | |authors=Pinho Nathalia, Wisniewski Jacek R, Dias-Lopes Geovane, Saboia-Vahia Leonardo, Souza Bombaca Ana Cristina, Mesquita-Rodrigues Camila, Menna-Barreto Rubem, Cupolillo Elisa, de Jesus Jose Batista, Padron Gabriel, Cuervo Patricia |
Latest revision as of 18:17, 6 November 2020
Pinho N, Wiลniewski JR, Dias-Lopes G, Saboia-Vahia L, Bombaรงa ACS, Mesquita-Rodrigues C, Menna-Barreto R, Cupolillo E, de Jesus JB, Padrรณn G, Cuervo P (2020) In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species. PLoS Negl Trop Dis 14:e0008509. |
Pinho Nathalia, Wisniewski Jacek R, Dias-Lopes Geovane, Saboia-Vahia Leonardo, Souza Bombaca Ana Cristina, Mesquita-Rodrigues Camila, Menna-Barreto Rubem, Cupolillo Elisa, de Jesus Jose Batista, Padron Gabriel, Cuervo Patricia (2020) PLoS Negl Trop Dis
Abstract: Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenic Leishmania spp. as well as hundreds of strains, but large-scale proteomics analyses did not follow these achievements and the Leishmania proteome remained mostly uncharacterized. Here, we report a comprehensive comparative study of the proteomes of strains representing L. braziliensis, L. panamensis and L. guyanensis species. Proteins extracted by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. "Total Protein Approach" and "Proteomic Ruler" were applied for absolute quantification of proteins. Principal component analysis demonstrated very high reproducibility among biological replicates and a very clear differentiation of the three species. Our dataset comprises near 7000 proteins, representing the most complete Leishmania proteome yet known, and provides a comprehensive quantitative picture of the proteomes of the three species in terms of protein concentration and copy numbers. Analysis of the abundance of proteins from the major energy metabolic processes allow us to highlight remarkably differences among the species and suggest that these parasites depend on distinct energy substrates to obtain ATP. Whereas L. braziliensis relies the more on glycolysis, L. panamensis and L. guyanensis seem to depend mainly on mitochondrial respiration. These results were confirmed by biochemical assays showing opposite profiles for glucose uptake and O2 consumption in these species. In addition, we provide quantitative data about different membrane proteins, transporters, and lipids, all of which contribute for significant species-specific differences and provide rich substrate for explore new molecules for diagnosing purposes. Data are available via ProteomeXchange with identifier PXD017696.
โข Bioblast editor: Plangger M
Labels: MiParea: Respiration, nDNA;cell genetics, Comparative MiP;environmental MiP
Pathology: Infectious
Organism: Protists Tissue;cell: Other cell lines Preparation: Intact cells
Pathway: ROX HRR: Oxygraph-2k
2020-08