Porter 2022 Abstract Bioblast: Difference between revisions
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== Affiliations and support == | == Affiliations and support == | ||
:::: Marilena Karavyraki | :::: Marilena Karavyraki(1), Erich Gnaiger(2), Richard K Porter(1) | ||
::::# School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected] | ::::# School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected] | ||
::::# Oroboros Instruments, Innsbruck, Austria | ::::# Oroboros Instruments, Innsbruck, Austria | ||
Revision as of 13:18, 30 May 2022
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Link: Bioblast 2022: BEC Inaugural Conference
Karavyraki Marilena, Gnaiger Erich, Porter Richard K (2022)
Event: Bioblast 2022
In an endeavour to understand the metabolic phenotype behind metastasis from oral squamous cell carcinomas, we characterised the bioenergetic profile of a human tongue derived cancer cell line (SCC-4 cells) and compared this profile to a pre-cancerous dysplastic oral keratinocyte (DOK) cell line also derived from human tongue. The human SCC-4 cancer cells had greater mitochondrial density but lower mitochondrial O2 flow per cell JO2 than DOK cells. The lower cell JO2 in SCC-4 cells can be partially explained by lower NADH-related enzymatic activity when compared to pre-cancerous DOK cells. In addition, SCC-4 cells have greater extracellular acidification rate (an index of glycolytic flux) when compared to DOK cells. In addition, treatment with recombinant human IL-6 (rhIL-6), known to drive anoikis resistance in SCC-4 cells but not DOK cells, impairs oxygen consumption in SCC-4 but not DOK cells, without affecting mitochondrial density. We conclude that SCC-4 cells have a less oxidative phenotype compared to DOK cells and that IL-6 attenuates mitochondrial function in SCC-4 cells while increasing glycolytic flux.
• Keywords: Oral Squamous Cancer Cells, Mitochondria, Interleukin 6, Dysplastic oral keratinocytes, Oxygen consumption • Bioblast editor: Plangger M
Affiliations and support
- Marilena Karavyraki(1), Erich Gnaiger(2), Richard K Porter(1)
- School of Biochemistry, Trinity Biomedical Science Institute, Trinity College Dublin, Ireland - [email protected]
- Oroboros Instruments, Innsbruck, Austria
- Marilena Karavyraki(1), Erich Gnaiger(2), Richard K Porter(1)
- Acknowledgements: Marie Curie Grant TRACT 721906 H2020-MCSA-ITN 2016; COST Action CA15203 MitoEAGLE (2016-2021).
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