Difference between revisions of "Ruthenium red"

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Ruthenium red

Description

Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca²⁺ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca²⁺-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].

Abbreviation: Rut

Reference: MiPNet03.02, Wikipedia

MitoPedia topics: Inhibitor

Application in HRR

Rut: Ruthenium Red (technical grade; [(NH₃)₅RuORu(NH₃)₄ORu(NH₃)₅]Cl₆); Sigma R 2751, 1 g; FW = 786.35.

Preparation of 10 mM stock solution (dissolved in H₂O):

1) Weigh 7.86 mg Ruthenium Red.

2) Dissolve in 1 ml H₂O.

3) Divide into 0.2 ml aliquots.

4) Store at -20 °C.

References

Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.

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 |mitopedia topic=Inhibitor
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-== References ==
+== Application in [[HRR]] ==
-[1] Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
+'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>);   Sigma R 2751, 1 g; FW = 786.35.
-[2] Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
+'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
+::1) Weigh 7.86 mg Ruthenium Red.
+::2) Dissolve in 1 ml H<sub>2</sub>O.
+::3) Divide into 0.2 ml aliquots.
+::4) Store at -20 °C.
+== References ==
+# Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
-== Application in [[HRR]] ==
+# Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
-'''Rut: Ruthenium Red''' (technical grade; [(NH<sub>3</sub>)<sub>5</sub>RuORu(NH<sub>3</sub>)<sub>4</sub>ORu(NH<sub>3</sub>)<sub>5</sub>]Cl<sub>6</sub>);   Sigma R 2751, 1 g; FW = 786.35
-'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
-::1) weigh 7.86 mg of Ruthenium Red
-::2) dissolve in 1 ml H<sub>2</sub>O
-::3) divide into 0.2 ml aliquots
-::4) store frozen at -20 °C