Difference between revisions of "SUIT-001 O2 ce-pce D003"
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|SUIT number=D03_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd | |SUIT number=D03_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd | ||
}} | }} | ||
::: [[MitoPedia: SUIT]] - '''[[SUIT reference protocol]] RP1''' | ::: [[MitoPedia: SUIT]] - '''[[SUIT reference protocol]] RP1''' | ||
::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNSGp(PGM) | ::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNSGp(PGM) | ||
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| sort=Was published in year | | sort=Was published in year | ||
| order=descending | | order=descending | ||
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{{MitoPedia concepts | |||
|mitopedia concept=SUIT protocol | |||
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{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
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Revision as of 10:03, 15 January 2019
Description
Abbreviation: FNSGp(Oct,PGM)
Reference: A: RP1 SUIT-001
SUIT number: D03_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
O2k-Application: O2
- MitoPedia: SUIT - SUIT reference protocol RP1
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: orthogonal 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp
Harmonized SUIT protocols
RP1 and RP2
- SUIT RP1:1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
- SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2
RP1 and RP2 in cells
RP1 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway states.
- SUIT RP1 in cells:ce1;1Dig;1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells:ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd
Harmonization between RP1 and RP2 in cells
RP1 and RP2 in PBMCs and PTLs
In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: SUIT 1 for PBMCs and PTLs
- SUIT RP1 for PBMCs and PLTs:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd
- SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2 in PBMC and PTL cells
References
MitoPedia concepts: SUIT protocol
MitoPedia methods:
Respirometry