SUIT-004 O2 pfi D010: Difference between revisions
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::: '''[[Mark names in DatLab]]:''' SUIT_NS(PM)01 | ::: '''[[Mark names in DatLab]]:''' SUIT_NS(PM)01 | ||
::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'' SUIT_NS(PM)01.xlsx | ::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'' SUIT_NS(PM)01.xlsx | ||
::::* Linked to [[SUIT RP1 | ::::* Linked to [[SUIT_FNSGp(PGM)01]] - SUIT RP1, specifically for human skeletal muscle mitochondria. | ||
::::* Harmonized with [[SUIT_FNS(PM)01]]. | ::::* Harmonized with [[SUIT_FNS(PM)01]]. | ||
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== Link to RP1 == | == Link to RP1 == | ||
:::: The '''SUIT_NS(PM)01''' protocol is linked to [[ | :::: The '''SUIT_NS(PM)01''' protocol is linked to [[SUIT_FNSGp(PGM)01]] for healthy human skeletal muscle mitochondria on the basis of the following rationale. | ||
[[File:FNSGp 1PM 2D 3c 4U 5G 6S 7Oct 8Rot 9Gp.jpg|right|300px|file= | [[File:FNSGp 1PM 2D 3c 4U 5G 6S 7Oct 8Rot 9Gp.jpg|right|300px|file=SUIT_FNSGp(PGM)01 |RP1]] | ||
::::# The first coupling control steps (1-4) are identical. | ::::# The first coupling control steps (1-4) are identical. | ||
Revision as of 07:25, 9 September 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-004 O2 pfi D010
Description
Abbreviation: NS_1PM 2D 3U 4S 5Rot
MitoPedia concepts:
SUIT protocol
- SUIT-catg: NS(PM)
- Subcatg: N+NS+S
- SUIT protocol pattern: orthogonal
- Mark names in DatLab: SUIT_NS(PM)01
- DatLab-Excel template: in prep. SUIT_NS(PM)01.xlsx
- Linked to SUIT_FNSGp(PGM)01 - SUIT RP1, specifically for human skeletal muscle mitochondria.
- Harmonized with SUIT_FNS(PM)01.
SUIT NS(PM)01-2c
File:NS 1PM 2D &c 3U 4S 5Rot.jpg
- NS_1PM 2D &c 3U 4S 5Rot
Link to RP1
- The SUIT_NS(PM)01 protocol is linked to SUIT_FNSGp(PGM)01 for healthy human skeletal muscle mitochondria on the basis of the following rationale.
- The first coupling control steps (1-4) are identical.
- RP1-5G is skipped, since PM and PGM yield nearly identical OXPHOS capacity. However, PM<PGM is a potential diagnostic for a defect in the PM-linked pathway upstream of CI.
- RP1-7Oct is skipped, since octanoylcarnitine exerts practically no stimulatory effect on OXPHOS capacity in state PMS or PGMS, but in conjunction with prolonged exposure may even yield a slight decline of respiration.
- RP1-9Gp is skipped, since glycerolphosphate exerts only a minor stimulatory effect on OXPHOS capacity in state SRot.
- Omission of the three steps described above shortens the SUIT protocol, thus reducing the risk of diminishing respiratory capacity over time, reducing the number of necessary reoxygenations, and providing more time for detailed evaluation of steady-states. The loss of diagnostic information may be minor relative to the benefits of simplification of the protocol, particularly in studies of exercise in healthy humans.
- RP1-10Ama may be skipped, since correction of the initial states by ROX measured in RP1-10 Ama may represent an over-correction, and inhibition takes a very long time in several cases (Pesta 2012 Methods Mol Biol).
- RP1-11Tm and RP1-12Azd are skipped, if a prolonged protocol is not practical due to limination of time.
