SUIT protocol library: Difference between revisions
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= Experimental details = | |||
== Oxygen saturation == | |||
== ADP kinetics == | == ADP kinetics == | ||
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[[#ETF -> CI -> CI+CII -> CII|ETF -> CI -> CI+CII -> CII]] | [[#ETF -> CI -> CI+CII -> CII|ETF -> CI -> CI+CII -> CII]] | ||
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= References and Notes = | |||
* [[List_of_respiratory_states]] | * [[List_of_respiratory_states]] |
Revision as of 08:19, 22 November 2011
This page is in preparation
Classes of protocols with coupling and substrate control
Linear
- Linear coupling control: at a single substrate state (classical State 3 -> State 4).
- Linear substrate control: at a single coupling state (unusual).
- Monodirectional linear coupling control: LN -> PD -> EF
- Bidirectional linear coupling control: LN -> PD -> LOmy -> EF
- CI
- CII
- CI+CII
- CIV
- ETF
- GPDH
Orthogonal
- Orthogonal coupling -> substrate control (mono- or bidirectional)
- Orthogonal substrate -> coupling control (mono- or bidirectional)
Diametral
- Diametral coupling -> substrate ->> control (mono- or bidirectional)
- Diametral substrate -> coupling ->> control (mono- or bidirectional)
CI -> CI+CII -> CII
Respiratory states: CIL -> CIP -> CI+IIP -> CI+IIE -> CIIE -> ROX
- GM+D+S+F+Rot Innsbruck-Copenhagen protocol: GM+D+c+S+F+Rot
- Cyt c: added after ADP; cyt c added after S; ADP conc: _ _; ROX: Ama (Boushel 2007 Diabetologia)
- PMG+D+S+F+Rot Brain protocol: PMG+D+c+S+F+Rot
- ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the OXPHOS state (CI+CII) is saturating (Sumbalova 2011 Abstract Bordeaux); ROX: Mna+Ama; Myx
CI -> CI -> CI+CII -> CII
- GM+D+P+S+F+Rot Innsbruck-Liege protocol 1: GM+D+c+P+S+F+Rot
- Cytochrome c: early addition of c ensures comparability of all states in case of any c effect.
- PM+D+G+S+F+Rot Innsbruck-Liege protocol 2: PM+D+c+G+S+F+Rot
ETF -> CI -> CI+CII -> CII
Respiratory states: ETFL -> ETFP -> CIP -> CI+IIP -> CI+IIE -> CIIE -> ROX
- OctM+D+G+S+F+Rot Innsbruck training protocol: OctM+D+G+c+S+F+Rot
Respiratory states: ETFL -> ETFP -> CIP -> CI+IIP -> CIIP -> CIIL -> CIIE -> ROX
- OctM+D+G+S+Rot+Omy+F Greenland-Monte Rosa protocol: OctM+D+G+S+Rot+Omy+F+c
- Cyt c: added after F. The absence of a significant c-effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation in the O2k (MiR05); Oxygen: oxygen limitation of flux was preventd by maintaining oxygen levels in the respirometer above air saturation (Pesta 2012 Methods Mol Biol); ROX: Mna+Ama
Experimental details
Oxygen saturation
ADP kinetics
Cytochrome c