Sarti 2013 Int J Mol Sci: Difference between revisions
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{{Publication | {{Publication | ||
|title=Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M (2013) New evidence for cross talk between melatonin and mitochondria mediated by a circadian-compatible interaction with nitric oxide. Int J Mol Sci 14:11259-76. | |title=Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M (2013) New evidence for cross talk between melatonin and mitochondria mediated by a circadian-compatible interaction with nitric oxide. Int J Mol Sci 14:11259-76. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/23759982 PMID: 23759982] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/23759982 PMID: 23759982 Open Access] | ||
|authors=Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M | |authors=Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M | ||
|year=2013 | |year=2013 | ||
|journal=Int J Mol Sci | |journal=Int J Mol Sci | ||
|abstract=Extending our previous observations, we have shown on HaCat cells that melatonin, at ~10-9 M concentration, transiently raises not only the expression of the neuronal nitric oxide synthase (nNOS) mRNA, but also the nNOS protein synthesis and the nitric oxide oxidation products, nitrite and nitrate. Interestingly, from the cell bioenergetic point of view, the activated NO-related chemistry induces a mild decrease of the oxidative phosphorylation (OXPHOS) efficiency, paralleled by a depression of the mitochondrial membrane potential. The OXPHOS depression is apparently balanced by glycolysis. The mitochondrial effects described have been detected only at nanomolar concentration of melatonin and within a time window of a few hours' incubation; both findings compatible with the melatonin circadian cycle. | |abstract=Extending our previous observations, we have shown on HaCat cells that melatonin, at ~10-9 M concentration, transiently raises not only the expression of the neuronal nitric oxide synthase (nNOS) mRNA, but also the nNOS protein synthesis and the nitric oxide oxidation products, nitrite and nitrate. Interestingly, from the cell bioenergetic point of view, the activated NO-related chemistry induces a mild decrease of the oxidative phosphorylation (OXPHOS) efficiency, paralleled by a depression of the mitochondrial membrane potential. The OXPHOS depression is apparently balanced by glycolysis. The mitochondrial effects described have been detected only at nanomolar concentration of melatonin and within a time window of a few hours' incubation; both findings compatible with the melatonin circadian cycle. | ||
|keywords=Nitric oxide; Cell bioenergetics; Respiratory chain; Circadian rhythm; Cell culture; Warburg effect; Reactive oxygen; Nitrogen species | |||
|mipnetlab=IT Roma Sarti P | |mipnetlab=IT Roma Sarti P | ||
}} | }} |
Revision as of 15:47, 30 April 2015
Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M (2013) New evidence for cross talk between melatonin and mitochondria mediated by a circadian-compatible interaction with nitric oxide. Int J Mol Sci 14:11259-76. |
Sarti P, Magnifico MC, Altieri F, Mastronicola D, Arese M (2013) Int J Mol Sci
Abstract: Extending our previous observations, we have shown on HaCat cells that melatonin, at ~10-9 M concentration, transiently raises not only the expression of the neuronal nitric oxide synthase (nNOS) mRNA, but also the nNOS protein synthesis and the nitric oxide oxidation products, nitrite and nitrate. Interestingly, from the cell bioenergetic point of view, the activated NO-related chemistry induces a mild decrease of the oxidative phosphorylation (OXPHOS) efficiency, paralleled by a depression of the mitochondrial membrane potential. The OXPHOS depression is apparently balanced by glycolysis. The mitochondrial effects described have been detected only at nanomolar concentration of melatonin and within a time window of a few hours' incubation; both findings compatible with the melatonin circadian cycle. โข Keywords: Nitric oxide; Cell bioenergetics; Respiratory chain; Circadian rhythm; Cell culture; Warburg effect; Reactive oxygen; Nitrogen species
โข O2k-Network Lab: IT Roma Sarti P
Labels: MiParea: Respiration, Pharmacology;toxicology
Organism: Human
Regulation: mt-Membrane potential Coupling state: OXPHOS
HRR: Oxygraph-2k