Sauer 1973 FEBS Lett: Difference between revisions

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Sauer LA (1973) Mitochondrial NAD-dependent malic enzyme: a new regulatory enzyme. FEBS Lett 33: 251-5.

Abstract:

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Made history

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 {{Publication
 |title=Sauer LA (1973) Mitochondrial NAD-dependent malic enzyme: a new regulatory enzyme. FEBS Lett 33: 251-5.
 |info=[http://www.ncbi.nlm.nih.gov/pubmed/4147096 PMID: 4147096 Open Access]
 |authors=Sauer LA
 |year=1973
 |journal=FEBS Lett
-|abstract=Electrophoretic evidence for multimolecular forms of malic enzyme (EC 1.1.1.40, NADP-malic dehydrogenase, decarboxylating) was reported by Henderson in 1966 [ 1]. Subsequent purification and assay by spectrophotometric and other methods revealed that malic enzyme isoenzymes were present in cytosol and mitochondrial fractions derived from several different animal tissues [2-61. Of particular importance were the recent findings of Frenkel [7] that the mitochondrial isoenzyme has allosteric properties, and that succinate was a positive modulator. Here, we demonstrate that calf adrenal cortex mitochondria contain an NAD-dependent malic enzyme (EC 1.1.1.39) with regulatory properties. Fumarate was a positive and ATP a negative modulator. The enzyme also reduced NADP but only at about one-quarter of the rate of NAD reduction. These kinetic properties suggest that this enzyme is an important regulator of the rate of malate oxidation.
 }}
 {{Labeling
-|organism=Bovines
+|additional=Made history
-|tissues=Nervous system
-|preparations=Enzyme
-|additional=Malic enzyme, Made history
 }}