Difference between revisions of "Schiffer 2018 PLoS One"
(Created page with "{{Publication |title=Schiffer TA, Christensen M, Gustafsson H, Palm F (2018) The effect of inactin on kidney mitochondrial function and production of reactive oxygen species....") Β |
Β |
||
(One intermediate revision by one other user not shown) | |||
Line 16: | Line 16: | ||
|preparations=Isolated mitochondria | |preparations=Isolated mitochondria | ||
|enzymes=Uncoupling protein | |enzymes=Uncoupling protein | ||
|topics=Oxygen kinetics | |||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
|pathways=N, NS | |pathways=N, NS | ||
|instruments=Oxygraph-2k, O2k-Fluorometer | |instruments=Oxygraph-2k, O2k-Fluorometer | ||
|additional= | |additional=2018-12, AmR, | ||
}} | }} |
Latest revision as of 13:32, 7 March 2020
Schiffer TA, Christensen M, Gustafsson H, Palm F (2018) The effect of inactin on kidney mitochondrial function and production of reactive oxygen species. PLoS One 13:e0207728. |
Schiffer TA, Christensen M, Gustafsson H, Palm F (2018) PLoS One
Abstract: Inactin is a long lasting anesthetic agent commonly used in rat studies, but is also shown to exert physiological effects such as reducing renal blood flow, glomerular filtration rate and depressing tubular transport capacity. The effect of inactin on isolated kidney mitochondria is unknown and may be important when studying related topics in anaesthetized animals. The aim of this study was to determine whether inactin exerts effects on mitochondrial function and production of reactive oxygen species. Kidney mitochondrial function and production of reactive oxygen after acutely (5 min) or longer (1.5 hour) anesthetizing rats with inactin was evaluated using high-resolution respirometry. The results demonstrate that inactin significantly improves respiratory control ratio, inhibits complex I in the mitochondrial respiratory chain, reduce both unregulated proton leak and time dependently reduce the regulated proton leak via uncoupling protein-2 and adenine nucleotide translocase. Inactin also contributes to increased mitochondrial hydrogen peroxide production. In conclusion, inactin exerts persistent effects on mitochondrial function and these profound effects on mitochondrial function should to be considered when studying mitochondria isolated from animals anesthesized with inactin.
β’ Bioblast editor: Plangger M β’ O2k-Network Lab: SE Uppsala Liss P
Labels: MiParea: Respiration, Pharmacology;toxicology
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Kidney Preparation: Isolated mitochondria Enzyme: Uncoupling protein Regulation: Oxygen kinetics Coupling state: LEAK, OXPHOS Pathway: N, NS HRR: Oxygraph-2k, O2k-Fluorometer
2018-12, AmR