Schwerzmann 1986 J Cell Biol: Difference between revisions
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::::* At 7Β·10<sup>9</sup> to 9Β·10<sup>9</sup> mitochondria per mg mt-protein, and 0.27 Β΅m<sup>3</sup> per mitochondrion, the volume per mitochondrion is 1.9 to 2.4 Β΅L/mg mt-protein. | ::::* At 7Β·10<sup>9</sup> to 9Β·10<sup>9</sup> mitochondria per mg mt-protein, and 0.27 Β΅m<sup>3</sup> per mitochondrion, the volume per mitochondrion is 1.9 to 2.4 Β΅L/mg mt-protein. | ||
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Latest revision as of 18:00, 16 January 2021
Schwerzmann K, Cruz-Orive LM, Eggman R, SΓ€nger A, Weibel ER (1986) Molecular architecture of the inner membrane of mitochondria from rat liver: a combined biochemical and stereological study. J Cell Biol 102:97-103. |
Schwerzmann K, Cruz-Orive LM, Eggman R, Saenger A, Weibel ER (1986) J Cell Biol
Abstract: The molecular structure of mitochondria and their inner membrane has been studied using a combined approach of stereology and biochemistry. The amount of mitochondrial structures (volume, number, surface area of inner membrane) in a purified preparation of mitochondria from rat liver was estimated by stereological procedures. In the same preparation, the oxidative activity of the respiratory chain with different substrates and the concentration of the redox complexes were measured by biochemical means. By relating the stereological and biochemical data, it was estimated that the individual mitochondrion isolated from rat liver has a volume of 0.27 Β΅m3, an inner membrane area of 6.5 Β΅m2, and contains between 2,600 (Complex I) and 15,600 (aa3) redox complexes which produce an electron flow of over 100,000 electrons per second with pyruvate as substrate. The individual redox complexes and the H+-ATPase together occur at a density of approximately 7,500/Β΅m2 and occupy approximately 40 % of the inner membrane area. From the respective densities it was concluded that the mean nearest distance between reaction partners is small enough (70-200 A) to cause the formation of micro-aggregates. The meaning of these results for the mechanism of mitochondrial energy transduction is discussed.
β’ Bioblast editor: Gnaiger E
- At 7Β·109 to 9Β·109 mitochondria per mg mt-protein, and 0.27 Β΅m3 per mitochondrion, the volume per mitochondrion is 1.9 to 2.4 Β΅L/mg mt-protein.
Cited by
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
Labels: MiParea: Respiration, mt-Structure;fission;fusion, mt-Membrane
Organism: Rat
Preparation: Isolated mitochondria Enzyme: Complex I, Complex IV;cytochrome c oxidase
Coupling state: OXPHOS Pathway: N
BEC 2020.2