Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Sharpe 2021 Front Oncol

From Bioblast
Revision as of 05:45, 6 February 2024 by Gnaiger Erich (talk | contribs) (Created page with "{{Publication |title=Sharpe MA, Baskin DS, Pichumani K, Ijare OB, Helekar SA (2021) Rotating magnetic fields inhibit mitochondrial respiration, promote oxidative stress and pr...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Publications in the MiPMap
Sharpe MA, Baskin DS, Pichumani K, Ijare OB, Helekar SA (2021) Rotating magnetic fields inhibit mitochondrial respiration, promote oxidative stress and produce loss of mitochondrial integrity in cancer cells. Front Oncol 11:768758. https://doi.org/10.3389/fonc.2021.768758

» PMID: 34858847 Open Access

Sharpe MA, Baskin DS, Pichumani K, Ijare OB, Helekar SA (2021) Front Oncol

Abstract: Electromagnetic fields (EMF) raise intracellular levels of reactive oxygen species (ROS) that can be toxic to cancer cells. Because weak magnetic fields influence spin state pairing in redox-active radical electron pairs, we hypothesize that they disrupt electron flow in the mitochondrial electron transport chain (ETC). We tested this hypothesis by studying the effects of oscillating magnetic fields (sOMF) produced by a new noninvasive device involving permanent magnets spinning with specific frequency and timing patterns. We studied the effects of sOMF on ETC by measuring the consumption of oxygen (O2) by isolated rat liver mitochondria, normal human astrocytes, and several patient derived brain tumor cells, and O2 generation/consumption by plant cells with an O2 electrode. We also investigated glucose metabolism in tumor cells using 1H and 13C nuclear magnetic resonance and assessed mitochondrial alterations leading to cell death by using fluorescence microscopy with MitoTracker™ and a fluorescent probe for Caspase 3 activation. We show that sOMF of appropriate field strength, frequency, and on/off profiles completely arrest electron transport in isolated, respiring, rat liver mitochondria and patient derived glioblastoma (GBM), meningioma and diffuse intrinsic pontine glioma (DIPG) cells and can induce loss of mitochondrial integrity. These changes correlate with a decrease in mitochondrial carbon flux in cancer cells and with cancer cell death even in the non-dividing phase of the cell cycle. Our findings suggest that rotating magnetic fields could be therapeutically efficacious in brain cancers such as GBM and DIPG through selective disruption of the electron flow in immobile ETC complexes.

Bioblast editor: Gnaiger E


Labels: MiParea: Respiration, Instruments;methods  Pathology: Cancer  Stress:Oxidative stress;RONS  Organism: Rat, Plants  Tissue;cell: Nervous system, Liver, Neuroblastoma  Preparation: Isolated mitochondria 

Regulation: Coupling efficiency;uncoupling  Coupling state: LEAK  Pathway:


Quantum biology, PhotoBiology