Sobotka 2015 Physiol Res: Difference between revisions
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|abstract=We compared the effect of alpha-tocopheryl succinate (TOS) on succinate-dependent respiration in rat liver mitochondria, homogenate and permeabilized hepatocytes in both a coupled and uncoupled state. In isolated mitochondria, a significant inhibitory effect was observed at a concentration of 5 microM, in liver homogenate at 25 microM and in permeabilized hepatocytes at 50 microM. The inhibitory effect of TOS on succinate respiration in an uncoupled state was less pronounced than in a coupled state in all the experimental models tested. When the concentration dependence of the TOS inhibitory effect was tested, the most sensitive in both states were isolated mitochondria; the most resistant were permeabilized hepatocytes. | |abstract=We compared the effect of alpha-tocopheryl succinate (TOS) on succinate-dependent respiration in rat liver mitochondria, homogenate and permeabilized hepatocytes in both a coupled and uncoupled state. In isolated mitochondria, a significant inhibitory effect was observed at a concentration of 5 microM, in liver homogenate at 25 microM and in permeabilized hepatocytes at 50 microM. The inhibitory effect of TOS on succinate respiration in an uncoupled state was less pronounced than in a coupled state in all the experimental models tested. When the concentration dependence of the TOS inhibitory effect was tested, the most sensitive in both states were isolated mitochondria; the most resistant were permeabilized hepatocytes. | ||
|keywords=Tocopheryl succinate, Complex II, Liver, Mitochondria, Homogenate, Hepatocytes | |keywords=Tocopheryl succinate, Complex II, Liver, Mitochondria, Homogenate, Hepatocytes | ||
|mipnetlab=CZ Hradec Kralove Cervinkova Z | |mipnetlab=CZ Hradec Kralove Cervinkova Z, CZ Prague Houstek J | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|tissues=Liver | |tissues=Liver | ||
|preparations=Permeabilized cells, Homogenate, Isolated mitochondria | |preparations=Permeabilized cells, Homogenate, Isolated mitochondria | ||
|couplingstates=OXPHOS, | |couplingstates=OXPHOS, ET | ||
| | |pathways=S | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2016-03 | |additional=2016-03 | ||
}} | }} |
Latest revision as of 16:09, 13 November 2017
Sobotka O, Drahota Z, KuΔera O, Endlicher R, RauchovΓ‘ H, ΔervinkovΓ‘ Z (2015) The effect of Ξ±-tocopheryl succinate on succinate respiration in rat liver mitochondria. Physiol Res 64 Suppl 5:S609-15. |
Sobotka O, Drahota Z, Kucera O, Endlicher R, Rauchova H, Cervinkova Z (2015) Physiol Res
Abstract: We compared the effect of alpha-tocopheryl succinate (TOS) on succinate-dependent respiration in rat liver mitochondria, homogenate and permeabilized hepatocytes in both a coupled and uncoupled state. In isolated mitochondria, a significant inhibitory effect was observed at a concentration of 5 microM, in liver homogenate at 25 microM and in permeabilized hepatocytes at 50 microM. The inhibitory effect of TOS on succinate respiration in an uncoupled state was less pronounced than in a coupled state in all the experimental models tested. When the concentration dependence of the TOS inhibitory effect was tested, the most sensitive in both states were isolated mitochondria; the most resistant were permeabilized hepatocytes. β’ Keywords: Tocopheryl succinate, Complex II, Liver, Mitochondria, Homogenate, Hepatocytes
β’ O2k-Network Lab: CZ Hradec Kralove Cervinkova Z, CZ Prague Houstek J
Labels: MiParea: Respiration, Pharmacology;toxicology
Organism: Rat
Tissue;cell: Liver
Preparation: Permeabilized cells, Homogenate, Isolated mitochondria
Coupling state: OXPHOS, ET
Pathway: S
HRR: Oxygraph-2k
2016-03