Difference between revisions of "Sodium orthovanadate"
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(Created page with "{{MitoPedia |abbr=VO4 |description='''Sodium vanadate (Na<sub>3</sub>VO<sub>4</sub>)''' is used as an ATPase inhibitor. }} __TOC__ == Application in HRR == {{Chemical_de...") |
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
{{Chemical_description | {{Chemical_description | ||
|abbr=VO4 | |abbr=VO4 | ||
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|complete name=Sodium vanadate | |complete name=Sodium vanadate | ||
|chem formula=Na<sub>3</sub>VO<sub>4</sub> | |chem formula=Na<sub>3</sub>VO<sub>4</sub> | ||
|molar mass= | |molar mass= 183.91 | ||
|vendor= | |vendor= Sigma-Aldrich | ||
|product number= | |product number= S6508 | ||
|store at= | |store at=RT | ||
|sensitivity= | |sensitivity= | ||
|cas= | |cas= | ||
Line 21: | Line 20: | ||
|h info= | |h info= | ||
}} | }} | ||
:::: '''Preparation of 25 mM stock solution''' (dissolved in H<sub>2</sub>O) | :::: '''Preparation of 25 mM stock solution''' (dissolved in H<sub>2</sub>O) | ||
::::# Weigh 45.98 mg. | |||
::::# Under a fume hood, dissolve in a volume of distilled H2O lower than 10 mL, using a borosilicate Erlenmeyer flask or Falcon tube. | |||
::::# Under the fume hood, adjust the pH of the solution to approximately 8.7 with HCl solution using pH test strips, the solution will turn yellow. | |||
::::# Prepare a heated bath under the fume hood and allow the water to boil (e.g., with a heating plate and a borosilicate glass flask with water). | |||
::::# Heat the solution in this bath until it turns colorless and allow it to cool to room temperature (it is possible to use an ice bath to make it faster). | |||
::::# Readjust the pH to 8.7 with HCl under the fume hood, the solution will turn yellow again. | |||
::::# Repeat this cycle of heating and readjusting the pH until the solution remains colorless at pH 8.7 and room temperature. | |||
::::# Adjust the volume with distilled H2O to 10 mL. | |||
::::# Divide in aliquots. | |||
::::# Store at +4 °C. | |||
:::: “This treatment removes all decavanadate ions present in the Na3VO4 solution, which induces mitochondrial membrane depolarization and inhibition of oxygen consumption”, [[Chinopoulos 2014 Methods Enzymol]] | |||
:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 2.4 µL using a 10 µL syringe (2 mL O2k-chamber). | |||
::::* Final concentration: 30 µM. | |||
== References == | == References == | ||
::::* [ | ::::* [Chinopoulos 2014 Methods Enzymol] | ||
{{MitoPedia concepts}} | {{MitoPedia concepts}} |
Revision as of 12:56, 26 April 2024
Description
Sodium vanadate (Na3VO4) is used as an ATPase inhibitor.
Abbreviation: VO4
Application in HRR
- VO4: orthovanadate (Sodium vanadate; Na3VO4), Sigma-Aldrich: S6508, store at RT, M = 183.91 g·mol-1
- Preparation of 25 mM stock solution (dissolved in H2O)
- Weigh 45.98 mg.
- Under a fume hood, dissolve in a volume of distilled H2O lower than 10 mL, using a borosilicate Erlenmeyer flask or Falcon tube.
- Under the fume hood, adjust the pH of the solution to approximately 8.7 with HCl solution using pH test strips, the solution will turn yellow.
- Prepare a heated bath under the fume hood and allow the water to boil (e.g., with a heating plate and a borosilicate glass flask with water).
- Heat the solution in this bath until it turns colorless and allow it to cool to room temperature (it is possible to use an ice bath to make it faster).
- Readjust the pH to 8.7 with HCl under the fume hood, the solution will turn yellow again.
- Repeat this cycle of heating and readjusting the pH until the solution remains colorless at pH 8.7 and room temperature.
- Adjust the volume with distilled H2O to 10 mL.
- Divide in aliquots.
- Store at +4 °C.
- “This treatment removes all decavanadate ions present in the Na3VO4 solution, which induces mitochondrial membrane depolarization and inhibition of oxygen consumption”, Chinopoulos 2014 Methods Enzymol
- Preparation of 25 mM stock solution (dissolved in H2O)
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2.4 µL using a 10 µL syringe (2 mL O2k-chamber).
- Final concentration: 30 µM.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
References
- [Chinopoulos 2014 Methods Enzymol]
MitoPedia topics: Inhibitor