Difference between revisions of "Stepanova 2018 J Cereb Blood Flow Metab"
(7 intermediate revisions by 3 users not shown) | |||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Stepanova A, Konrad C, Guerrero-Castillo S, Manfredi G, Vannucci S, Arnold S, Galkin A (2018) Deactivation of mitochondrial complex I after hypoxia-ischemia in the immature brain. J Cereb Blood Flow Metab | |title=Stepanova A, Konrad C, Guerrero-Castillo S, Manfredi G, Vannucci S, Arnold S, Galkin A (2018) Deactivation of mitochondrial complex I after hypoxia-ischemia in the immature brain. J Cereb Blood Flow Metab 39:1790-802. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/29629602 PMID: 29629602] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/29629602 PMID: 29629602] | ||
|authors=Stepanova A, Konrad C, Guerrero-Castillo S, Manfredi G, Vannucci S, Arnold S, Galkin A | |authors=Stepanova A, Konrad C, Guerrero-Castillo S, Manfredi G, Vannucci S, Arnold S, Galkin A | ||
Line 10: | Line 10: | ||
|mipnetlab=US NY New York Galkin A, HU Budapest Chinopoulos C | |mipnetlab=US NY New York Galkin A, HU Budapest Chinopoulos C | ||
}} | }} | ||
== Cited by == | |||
{{Template:Cited by Komlodi 2021 MitoFit AmR-O2}} | |||
{{Template:Cited by Komlodi 2021 MitoFit AmR}} | |||
{{Template:Cited by Komlodi 2022 MitoFit ROS review}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
Line 21: | Line 25: | ||
|pathways=N, S, ROX | |pathways=N, S, ROX | ||
|instruments=Oxygraph-2k, O2k-Fluorometer | |instruments=Oxygraph-2k, O2k-Fluorometer | ||
|additional=2018-09, | |additional=2018-09, AmR, Tissue normoxia, MitoFit 2021 AmR, MitoFit 2021 AmR-O2, MitoFit 2022 ROS review | ||
}} | }} |
Latest revision as of 21:42, 13 April 2022
Stepanova A, Konrad C, Guerrero-Castillo S, Manfredi G, Vannucci S, Arnold S, Galkin A (2018) Deactivation of mitochondrial complex I after hypoxia-ischemia in the immature brain. J Cereb Blood Flow Metab 39:1790-802. |
Stepanova A, Konrad C, Guerrero-Castillo S, Manfredi G, Vannucci S, Arnold S, Galkin A (2018) J Cereb Blood Flow Metab
Abstract: Mortality from perinatal hypoxic-ischemic (HI) brain injury reached 1.15 million worldwide in 2010 and is also a major factor for neurological disability in infants. HI directly influences the oxidative phosphorylation enzyme complexes in mitochondria, but the exact mechanism of HI-reoxygenation response in brain remains largely unresolved. After induction of HI-reoxygenation in postnatal day 10 rats, activities of mitochondrial respiratory chain enzymes were analysed and complexome profiling was performed. The effect of conformational state (active/deactive (A/D) transition) of mitochondrial complex I on H2O2 release was measured simultaneously with mitochondrial oxygen consumption. In contrast to cytochrome c oxidase and succinate dehydrogenase, HI-reoxygenation resulted in inhibition of mitochondrial complex I at 4 h after reoxygenation. Immediately after HI, we observed a robust increase in the content of deactive (D) form of complex I. The D-form is less active in reactive oxygen species (ROS) production via reversed electron transfer, indicating the key role of the deactivation of complex I in ischemia/reoxygenation. We describe a novel mechanism of mitochondrial response to ischemia in the immature brain. HI induced a deactivation of complex I in order to reduce ROS production following reoxygenation. Delayed activation of complex I represents a novel mitochondrial target for pathological-activated therapy. • Keywords: A/D transition, Ischemia, Immature brain, Mitochondrial complex I, Reactive oxygen species • Bioblast editor: Plangger M • O2k-Network Lab: US NY New York Galkin A, HU Budapest Chinopoulos C
Cited by
- Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi:10.26124/BEC:2021-0004
- Komlódi T, Schmitt S, Zdrazilova L, Donnelly C, Zischka H, Gnaiger E. Oxygen dependence of hydrogen peroxide production in isolated mitochondria and permeabilized cells. MitoFit Preprints (in prep).
- Komlódi T, Gnaiger E (2022) Discrepancy on oxygen dependence of mitochondrial ROS production - review. MitoFit Preprints 2022 (in prep).
Labels: MiParea: Respiration
Stress:Ischemia-reperfusion, Hypoxia Organism: Rat Tissue;cell: Nervous system Preparation: Isolated mitochondria Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Supercomplex Regulation: Oxygen kinetics Coupling state: LEAK, OXPHOS, ET Pathway: N, S, ROX HRR: Oxygraph-2k, O2k-Fluorometer
2018-09, AmR, Tissue normoxia, MitoFit 2021 AmR, MitoFit 2021 AmR-O2, MitoFit 2022 ROS review