Difference between revisions of "TPP+ inhibitory effect"
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|description=A major task in establishing a procedure for measurement of [[mitochondrial membrane potential]] using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The '''TPP<sup>+</sup> inhibitory effect''' (this also applies to TPMP<sup>+</sup> and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP<sup>+</sup>, and for restriction of experimental TPP<sup>+</sup> concentrations below the inhibitory range. | |description=A major task in establishing a procedure for measurement of [[mitochondrial membrane potential]] using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The '''TPP<sup>+</sup> inhibitory effect''' (this also applies to TPMP<sup>+</sup> and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP<sup>+</sup>, and for restriction of experimental TPP<sup>+</sup> concentrations below the inhibitory range. | ||
|info=[[MiPNet14.05]] | |info=[[MiPNet14.05]] | ||
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{{MitoPedia concepts}} | |||
{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
}} | }} | ||
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{{Template:Keywords Membrane potential}} | {{Template:Keywords Membrane potential}} | ||
Revision as of 09:27, 10 September 2018
- high-resolution terminology - matching measurements at high-resolution
TPP+ inhibitory effect
Description
A major task in establishing a procedure for measurement of mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The TPP+ inhibitory effect (this also applies to TPMP+ and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range.
Reference: MiPNet14.05
MitoPedia methods: Respirometry
MitoPedia topics: Inhibitor
