Difference between revisions of "TPP+ inhibitory effect"
From Bioblast
Line 3: | Line 3: | ||
|info=[[MiPNet14.05]] | |info=[[MiPNet14.05]] | ||
}} | }} | ||
{{ | {{Keywords Force and membrane potential}} | ||
{{MitoPedia methods | {{MitoPedia methods |
Revision as of 11:03, 3 January 2019
Description
A major task in establishing a procedure for measurement of mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The TPP+ inhibitory effect (this also applies to TPMP+ and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range.
Reference: MiPNet14.05
Template:Keywords Force and membrane potential
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor