TPP+ inhibitory effect: Difference between revisions

Latest revision as of 00:03, 18 February 2020

high-resolution terminology - matching measurements at high-resolution

TPP+ inhibitory effect

Description

A major task in establishing a procedure for measurement of mitochondrial membrane potential using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The TPP⁺ inhibitory effect (this also applies to TPMP⁺ and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP⁺, and for restriction of experimental TPP⁺ concentrations below the inhibitory range.

Reference: MiPNet14.05

Bioblast links: Force and membrane potential - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Fundamental relationships

» Force

» Affinity

» Flux

» Advancement

» Advancement per volume

» Stoichiometric number

mt-Membrane potential and protonmotive force

» Protonmotive force

» Mitochondrial membrane potential

» Chemical potential

» Faraday constant

» Format

» Uncoupler

O2k-Potentiometry

» O2k-Catalogue: O2k-TPP+ ISE-Module

» O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE

» TPP - O2k-Procedures: Tetraphenylphosphonium

» Specifications: MiPNet15.08 TPP electrode

» Poster

» Unspecific binding of TPP+

» TPP+ inhibitory effect

O2k-Fluorometry

» O2k-Catalogue: O2k-FluoRespirometer

» O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual

» Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin

» TMRM - O2k-Procedures: TMRM

O2k-Publications

» O2k-Publications: mt-Membrane potential

» O2k-Publications: Coupling efficiency;uncoupling

MitoPedia methods: Respirometry

MitoPedia topics: Inhibitor

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-{{Technical support without banner}}
+{{MitoPedia
+|description=A major task in establishing a procedure for measurement of [[mitochondrial membrane potential]] using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. The '''TPP<sup>+</sup> inhibitory effect''' (this also applies to TPMP<sup>+</sup> and other indicator molecules) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP<sup>+</sup>, and for restriction of experimental TPP<sup>+</sup> concentrations below the inhibitory range.
+|info=[[MiPNet14.05]]
+}}
+{{Keywords: Force and membrane potential}}
+{{MitoPedia methods
-{{MitoPedia
+|mitopedia method=Respirometry
-|description=A major first task in establishing a system for measurement of [[Mitochondrial membrane potential]] using probe molecules is the evaluation of inhibitory concentrations of the probe molecule on the activity of respiration. This important 'side effect' of TPP+ and TPMP+ (and various dyes) is frequently ignored. Accurate knowledge of a threshold concentration is required to evaluate the necessary limit of detection of TPP+, and for restriction of experimental TPP+ concentrations below the inhibitory range [[MiPNet14.05]].
-|info=MiPNet14.05
-|type=Respiration
 }}
-{{Labeling
+{{MitoPedia topics
-|instruments=MultiSensor pH; TPP; NO, Chemicals; Media
+|mitopedia topic=Inhibitor
-|topics=Respiration; OXPHOS; ETS Capacity
 }}
-[http://www.oroboros.at/index.php?id=protocols_tpp-membranepotential MiPNet14.05]