Talk:Glucose

MiPNet discussion forum: glucose oxidation in skeletal muscle (2013-10-29)

Dominik Pesta

Has anyone ever looked at glucose oxidation in intact skeletal muscle fibres (of mouse muscle)? I have tried with a Krebs Henseleit Buffer (no substrates) and Malate and/or Pyruvate followed by glucose (basal) and insulin titration (insulin stimulated) but do not get any stimulation of respiration. Although malate will not permeate the cell, I would at least expect some stimulation with glucose. Any thoughts on that?

Rob Wuest

My initial thought on Dominik's question was that the mitos are quite likely not stimulated well enough with insulin to respire any of the glucose. I suspect that the insulin titration is activating the "glucose-entering" pathway, but that pathway quite likely does not consume enough ATP in sk muscle to quickly activate any of the mitochondria. There is enough PCr etc to buffer all this. The easiest way to increase VO2 in these fibres is to stimulate them to contract.