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| == Leaky Chamber ==
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| === Leaky chamber test ===
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| :::* When the leakage is so little that cannot be noticed in a short amount of time, we recommend to follow the ''Leaky chamber test'' below:
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| ::::# Decrease the oxygen concentration down to ~ 50 ΞΌM (using [[Dithionite]] or N<sub>2</sub> injection)
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| ::::# Let the O2k record overnight with the [[closed chamber|chamber closed]] and illumination switched off
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| ::::# The oxygen concentration should decrease overtime due to the O<sub>2</sub> consumed by [[OroboPOS]] as it is shown in the figure. In this case, the chamber is assembledΒ properly.
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| ::::# If the oxygen concentration increases over time, a reassemble of the chamber is needed.
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| [[File:Leaking_chamber_2020-02-03 P2-03.png|1500px]]
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| ::::*'''Text in discussion for the page:'''
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| ::::* To exclude the existence of an unnoticed leak from the chamber, we recommend to follow the ''Leaky chamber test'' below - especially after chamber reassembly:
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| ::::# Decrease the oxygen concentration down to ~ 50 ΞΌM (using [[Dithionite]] or N<sub>2</sub> injection).
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| ::::# Record the O2k measurement overnight with the [[closed chamber|chamber closed]] and illumination switched off.
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| ::::# The oxygen concentration should decrease overtime due to the O<sub>2</sub> consumed by [[OroboPOS]] as it is shown in the figure below. In this case, the chamber is assembledΒ properly.
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| ::::# If the oxygen concentration increases over time, a reassemble of the chamber is needed.
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| ::::[[User:CecattoC|CecattoC]] ([[User talk:CecattoC|talk]]) 15:52, 19 March 2020 (CET). So, it should be done every reassemble otherwise nobody will noticed that? How frequent you received cases with this or noticed leaky chamber in our lab?
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