Difference between revisions of "Talk:Ruthenium red"
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== MiPNet discussion forum: use of Ruthenium red (2013-11-28) == | == MiPNet discussion forum: use of Ruthenium red (2013-11-28) == | ||
=== Patrice | === Patrice Petit === | ||
We work with cells which we treat with pollutants that induce a shift in metabolic activity (i.e. modifying the glycolysis pathway and inhibiting the mitochondrial function. We tried to use Ruthenium red to target the mitochondrial calcium uniporter; however, we got the impression that the RR works, we have some evidence which makes us suspecting non-specific targeting of the RR. | We work with cells which we treat with pollutants that induce a shift in metabolic activity (i.e. modifying the glycolysis pathway and inhibiting the mitochondrial function. We tried to use Ruthenium red to target the mitochondrial calcium uniporter; however, we got the impression that the RR works, we have some evidence which makes us suspecting non-specific targeting of the RR. | ||
The question are: | The question are: | ||
Revision as of 10:25, 28 November 2013
MiPNet discussion forum: use of Ruthenium red (2013-11-28)
Patrice Petit
We work with cells which we treat with pollutants that induce a shift in metabolic activity (i.e. modifying the glycolysis pathway and inhibiting the mitochondrial function. We tried to use Ruthenium red to target the mitochondrial calcium uniporter; however, we got the impression that the RR works, we have some evidence which makes us suspecting non-specific targeting of the RR. The question are:
1 - does RR easily penetrate the plasma membrane (we can eventually add 0,001 % digitonine to our cells)?
2 - when measuring calcium with Fluo4 -AM we do not understand why RR totally abolish the fluorescence of the RR, since it is only the MUC which should be affected. Does RR non-specifically block other calcium pumps, channels and transport systems?
