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| {{MitoPedia
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| |abbr= CCP ce-pce S(Rot)
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| |description=[[File:Ce1;1Dig;1SRot;2D;(3Omy);4U;4Anoxia;5Ama.png|400px]]
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| |info='''A''' Coupling control protocol to analyze mitochondrial O<sub>2</sub> consumption and the redox state of the Q-pool in S(Rot)-pathway control state in permeabilized cells- '''[[SUIT-006]]'''
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| |application=Q
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| |SUIT number=D073_ce1;1Dig;1Q;1SRot;2D;(3Omy);4U;4Anoxia;5Ama
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| }}
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| {{Template:SUIT text D073}}
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| ''in preparation''
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| __TOC__
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| Communicated by [[Komlodi T]] (last update 2021-02-08)
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| == Representative traces ==
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| [[File:SUIT-006 Q ce-pce D073 traces O2.png|700px]]
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| [[File:SUIT-006 Q ce-pce D073 traces Q.png|700px]]
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| {{Template:SUIT-006 Q ce-pce D073}}
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| == Strengths and limitations ==
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| :::* SUIT-006 Q ce-pce D073 in combination with [[SUIT-031 Q ce-pce D074]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows:
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| :::: 1. to obtain reference values.
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| :::: 2. to evaluate mitochondrial physiological diversity, generating a mt-database on comparative mitochondrial physiology.
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| :::: 3. to screen specific defects.
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| :::* To study the effect of [[coenzyme Q2]] on mitochondrial respiration, the following protocol can be used in a parallel experiment after permeabilization of the plasma membrane with digitonin: [[SUIT-006 O2 mt D022]].
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| :::* Addition of Ama can be omitted if a sufficient sample amount is available to detect the fully reduced Q state.
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| :::+ Reasonable duration of the experiment.
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| :::- Omy concentration has to be determined if used. Higher concentrations of Omy may inhibit the ET state.
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| :::- CIV activity cannot be determined and cytochrome c test cannot be performed together with the [[Q-Sensor]].
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| :::+ Cytochrome c test can be performed in the following protocols after permeabilization of the plasma membrane with digitonin:[[SUIT-006 O2 mt D022]].
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| :::+ This protocol can be extended with the Complex IV module in the following protocols after permeabilization of the plasma membrane with digitonin: [[SUIT-006 O2 mt D022]].
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| :::- Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors.
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| :::- The concentration of the oxidized and reduced Q fraction cannot be determined.
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| == Compare SUIT protocols ==
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| :::* [[SUIT-006 O2 ce-pce D000]]: Coupling-control protocol for permeabilized cells.
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| :::* [[SUIT-031 Q ce-pce D074]]: Harmonized SUIT protocol.
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| == Chemicals and syringes ==
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| {{Template:Chemicals ce}}
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| {{Template:Chemicals Q}}
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| {{Template:Chemicals SUIT-006 Q mt D071}}
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| ::: Suggested stock concentrations are shown in the specific DL-Protocol.
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| == References ==
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| {{#ask:[[Category:Publications]] [[Additional label::SUIT-006 Q ce-pce D073]]
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| | ?Was published in year=Year
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| | ?Has title=Reference
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| | ?Mammal and model=Organism
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| | ?Tissue and cell=Tissue;cell
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| | format=broadtable
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| | limit=5000
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| | offset=0
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| | sort=Was published in year
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| | order=descending
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| }}
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| {{MitoPedia concepts
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| |mitopedia concept=SUIT protocol, SUIT A, Find
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| }}
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| {{MitoPedia methods
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| |mitopedia method=Respirometry
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| }}
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