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| | [[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]] |
| == Which meaning of 'uncoupling'? == | | == Popular Bioblast page == |
| | | ::: [[Uncoupler]] has been accessed more than |
| I was getting increasingly worried about the fact that students have difficulties to understanding [[uncoupling]] or [[coupling]] in bioenergetics and mitochondrial physiology. In this context we should realize that conventional terminology is not particularly helpful. In fact, the meaning of 'uncoupling' (in the current literature and bioenergetics textbooks) must usually be deduced from the specific context, but this can be achieved only after the concept and context are clear. Are intact mitochondria partially uncoupled, or partially coupled?
| | ::::* 50,000 times (2019-12-11) |
| | | ::::* 40,000 times (2018-11-22) |
| Exaggerating, we may be used to phrases such as:
| | ::::* 35,000 times (2017-02-05) |
| | | ::::* 30,000 times (2016-09-22) |
| *'''''Uncoupling''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''uncoupled''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''uncoupled''' respiration.'' | | ::::* 25,000 times (2016-04-23) |
| | | ::::* 20,000 times (2015-09-24) |
| Perhaps this is intuitively more clear:
| | ::::* 15,000 times (2014-12-22) |
| | | ::::* 10,000 times (2014-05-22) |
| *''Dyscoupling was studied in stressed cells by measurement of partially coupled'' '''[[ROUTINE]] respiration''''', evaluation of'' '''[[LEAK]] respiration''' ''after inhibition with oligomycin, and FCCP titration to quantify'' '''[[ETS]] capacity''' ''in the non-coupled state.'' | | ::::* 5,000 times (2013-04-18) |
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| I am even still worried about the different meanings of (1) intrinsic and (2) experimental uncoupling, but have not found a solution. The distinction between dyscoupling versus intrinsic (adaptive?) uncoupling is a fundamental challenge, not of terminology, but of understanding the physiological functions of intrinsic uncoupling.
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| --[[User:Gnaiger Erich|Gnaiger Erich]] 09:01, 31 May 2011 (CEST) | |
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| == Uncoupler titrations cause small jumps in oxygen concentration ==
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| * Q: When adding FCCP, the O2 concentration rises a bit. Does this mean that the cells are consuming or releasing oxygen?
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| * A: You add FCCP dissolved in ethanol. Ethanol has a high oxygen solubility, hence your titration is the cause of the increase in oxygen concentration. | |
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| == Uncoupler accessed ==
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| [[Uncoupler]] has been accessed more than
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| * 5,000 times (2013-04-18)
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