Template:AmR assay
From Bioblast
Steps and respiratory states
| Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
|---|---|---|---|---|
| 0DTPA |
DTPA is an iron chelator, which decreases the chemical fluorescence background created by the Amplex UltraRed assay. Administration of DTPA into the O2k-chamber is recommended before all other chemicals because the iron chelation capacity of the compound is time-dependent (approx. 10-15 min). However, the experiments can be carried out in the absence of DTPA. | |||
| 0SOD |
SOD or superoxide dismutase converts the anion superoxide released by the mitochondria into H2O2, making it accessible to the Amplex UltraRed assay. | |||
| 0HRP |
HRP or horseradish peroxidase catalyses the conversion of Amplex UltraRed and H2O2 towards the fluorescent resorufin. | |||
| 0AmR |
AmR or Amplex UltraRed forms resorufin with H2O2 catalysed by horseradish peroxidase HRP. |
