Template:Chemicals SUIT-006 Q mt D071: Difference between revisions
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| Line 5: | Line 5: | ||
| 1Rot | | 1Rot | ||
| {{Template:Rotenone}} | | {{Template:Rotenone}} | ||
| This step is essential to reach the fully oxidized | | This step is essential to reach the fully oxidized CoQ<sub>2</sub> needed to calculate the reduced Q fraction. | ||
|- | |- | ||
| 1S | | 1S | ||
| Line 21: | Line 21: | ||
| 4U | | 4U | ||
| {{Template:CCCP}} | | {{Template:CCCP}} | ||
| Can be substituted for other [[MitoPedia:_Uncouplers|uncoupler]] | | Can be substituted for other [[MitoPedia:_Uncouplers|uncoupler]]. | ||
|- | |- | ||
| | | 5Anox | ||
| | |||
| The O<sub>2</sub> concentration in the O2k-chamber can be decreased by N<sub>2</sub> or H<sub>2</sub> injection to reach faster anoxia, see: [[Setting the oxygen concentration]]. | |||
|- | |||
| 6Ama | |||
| {{Template:Antimycin A}} | | {{Template:Antimycin A}} | ||
| This step can be | | This step can be omitted. | ||
|} | |} | ||
Latest revision as of 16:23, 25 November 2021
| Step | Chemical(s) and link(s) | Comments |
|---|---|---|
| 1Rot | Rotenone (Rot) | This step is essential to reach the fully oxidized CoQ2 needed to calculate the reduced Q fraction. |
| 1S | Succinate (S) | |
| 2D | ADP (D) | |
| (3Omy) | Oligomycin (Omy) | This step can be skipped. |
| 4U | Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U) | Can be substituted for other uncoupler. |
| 5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
| 6Ama | Antimycin A (Ama) | This step can be omitted. |
