Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 12:33, 29 March 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 13

Mar-25 Mo

Living cells or intact cells?

Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.
- »Living cells«, »Gnaiger 2020 BEC Mitochondrial physiology«, »Gnaiger 2020 BEC MitoPathways«
- communicated by »Leo Elettra« and »Timon-Gomez Alba«

Mar-26 Tu

Mar-27 We

Mar-28 Th

"Introduction to the new models available with the NextGen was immensely helpful. This workshop has been critical for implementation of new techniques and advancement of my research."

Zunica Elizabeth, from the O2k-Network »US LA Baton Rouge Noland RC«
- »O2k-Feedback«

Mar-29 Fr

Next Week

2024 Week 14

Apr-01 Mo April - Parkinson's Disease Awareness Month

"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."

Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. »Bioblast link«

from the O2k-Network »US CA San Francisco Nussbaum RL« and »DE Frankfurt Eckert GP«

Apr-02 Tu

Apr-03 We

Apr-04 Th

"Why is it important to check the H₂O₂ sensitivity when conducting an Amplex UltraRed (AmR) assay?

The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H₂O₂ calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.

- »MiPNet24.10 H2O2 flux analysis«, »Amplex UltraRed«
- communicated by »Grings Mateus« and »Schmitt Sabine«

Apr-05 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 48
-*'''Nov- 27 Mo'''
+Week 13
-----
-*'''Nov- 28 Tu'''
+* '''Mar-25 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Protocols that SUIT your research question'''<br>
+|''' Living cells or intact cells? '''<br>
-Using '''S'''ubstrate-'''U'''ncoupler-'''I'''nhibitor-'''T'''itration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay. <br>
+Living cells are frequently labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.    <br>
-- »'''[https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocols]'''« and »'''[https://bioblast.at/index.php/SUITbrowser SUITbrowser]'''« <br>
+- »'''[[Living cells]]'''«, »'''[[BEC 2020.1|Gnaiger 2020 BEC Mitochondrial physiology]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''« <br>
-- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
+- communicated by »[[Leo Elettra]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Nov- 29 We'''
+* '''Mar-26 Tu'''
 ----
-*'''Nov- 30 Th'''
+* '''Mar-27 We'''
 ----
-*'''Dec- 01 Fr'''  <span style="color:red">World AIDS day</span>
+* '''Mar-28 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
-|'''"We therefore hypothesized that the susceptibility to HIV-1 infection of FOXO1-inhibited resting T cells could be due to an increased cell metabolism. (...) Results obtained by high-resolution respirometry experiments of [human peripheral blood T cells] PBT (...) showed that [ROUTINE] respiration at the steady state was increased by [FOXO1 inhibitor] AS1842856."''' <br>
+|''' ''"Introduction to the new models available with the NextGen was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my research."'''''<br>
-* Roux A, Leroy H, De Muylder B, Bracq L, Oussous S, Dusanter-Fourt I, Chougui G, Tacine R, Randriamampita C, Desjardins D, Le Grand R, Bouillaud F, Benichou S, Margottin-Goguet F, Cheynier R, Bismuth G, Mangeney M (2019) FOXO1 transcription factor plays a key role in T cell-HIV-1 interaction.  [[Roux 2019 PLoS Pathog|»Bioblast link«]]
+[[Zunica Elizabeth]], from the O2k-Network »[[US LA Baton Rouge Noland RC]]«<br>
-:from the O2k-Network »[[FR Paris Bouillaud F]]«
+- »'''[[O2k-Feedback]]'''«<br>
 |}
-:::: '''»[[AMI 2023 Jhansi IN]]«''' Dec 1-3
 ----
+* '''Mar-29 Fr'''
+----
+'''<big>Next Week</big>'''
+Week 14
-'''<big>Next Week</big>'''
+* '''Apr-01 Mo'''  April - Parkinson's Disease Awareness Month
-Week 49
-*'''Dec- 04 Mo'''
+:{|
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+|'''"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."''' <br>
+* Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. [[Gispert 2009 PLoS One|»Bioblast link«]]
+:from the O2k-Network »[[US CA San Francisco Nussbaum RL]]« and »[[DE Frankfurt Eckert GP]]«
+|}
+----
+* '''Apr-02 Tu'''
 ----
-*'''Dec- 05 Tu'''
+* '''Apr-03 We'''
+----
+* '''Apr-04 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' Is DatLab 8 compatible with your operative system?'''<br>
+|''' "Why is it important to check the H<sub>2</sub>O<sub>2</sub> sensitivity when conducting an Amplex UltraRed (AmR) assay?
-The DatLab 8 software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.
+The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H<sub>2</sub>O<sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br>
-<br>
-- »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
-|}
-----
+- »'''[[MiPNet24.10 H2O2 flux analysis]]'''«, »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Amplex UltraRed]'''«  <br>
-*'''Dec- 06 We'''
+- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
-:{|
-| width="100" align="center" |[[File:MitoFit Preprints.png|100px|link=https://wiki.oroboros.at/images/0/00/Cecatto_2023_MitoFit.pdf |MitoFit pdf]]
-|'''"The capacity of FA oxidative phosphorylation (F-OXPHOS) with palmitoylcarnitine was up to 4 times higher than respiration with octanoylcarnitine. The optimal concentration of palmitoylcarnitine was 10 μM which corresponds to the total concentration of LC acylcarnitines in the brain."'''<br>
-* Cecatto C, Cardoso LHD, Ozola M, Korzh S, Zvejniece L, Gukalova B, Doerrier C, Dambrova M, Gnaiger E, Makrecka-Kuka M, Liepinsh E (2023) Fatty acid β-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse, rat and Drosophila brain, ischemia and aging models. MitoFit Preprints 2023.10. https://doi.org/10.26124/mitofit:2023-0010
-:from the O2k-Network »[[AT Innsbruck Oroboros]]« and »[[LV Riga Liepins E]]«
 |}
 ----
-*'''Dec- 07 Th'''
+* '''Apr-05 Fr'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"(…) L-Erg [L-Ergothioneine] treatment prevented and delayed cystine lithiasis in a cystinuria mouse model by increasing urinary cystine solubility and restored kidney GSH content and OXPHOS function."''' <br>
-* Mayayo-Vallverdú C, López de Heredia M, Prat E, González L, Espino Guarch M, Vilches C, Muñoz L, Asensi MA, Serra C, Llebaria A, Casado M, Artuch R, Garrabou G, Garcia-Roves PM, Pallardó FV, Nunes V (2023) The antioxidant l-Ergothioneine prevents cystine lithiasis in the Slc7a9-/- mouse model of cystinuria [[Mayayo-Vallverdu 2023 Redox Biol|»Bioblast link«]]
-:from the O2k-Network »[[ES Barcelona Moren C]]« and »[[ES Barcelona Garcia-Roves PM]]«
-|}
-*'''Dec- 08 Fr'''
 ----