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Difference between revisions of "Template:Current week ecosystem agenda"

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
2023 Week 50


*'''Dec- 11 Mo''' - International Mountain Day
2024 Week 13
 
* '''Mar-25 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''Mountain slopes can be difficult, but O<sub>2</sub> slopes can be smooth with DatLab'''<br>
|''' Living cells or intact cells? '''<br>
The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.  
Living cells are frequently labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.   <br>
<br>
- »'''[[Living cells]]'''«, »'''[[BEC 2020.1|Gnaiger 2020 BEC Mitochondrial physiology]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''« <br>
- »'''[https://wiki.oroboros.at/index.php/Flux_/_Slope#Smoothing.2C_noise.2C_and_sample_stability Smoothing in Flux/Slope]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Timon-Gomez Alba]]«  
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
|}
|}
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*'''Dec- 12 Tu'''
* '''Mar-26 Tu'''  
:{|
| width="100" align="center" |[[File:MitoFit Preprints.png|100px|MitoFit pdf]]
| '''From preprint to Gold Open Access publication in J Biol Chem'''<br>
* Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. [[Gnaiger 2023 J Biol Chem|»Bioblast link«]]
|}
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*'''Dec- 13 We'''
* '''Mar-27 We'''  
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*'''Dec- 14 Th'''
* '''Mar-28 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
|'''"HCR [High capacity runner rats] showed a consistent and robust trend of higher maximal oxygen consumption compared to LCR [low capacity runner rats], especially for long chain FAs [fatty acids]."''' <br>
|''' ''"Introduction to the new models available with the NextGen was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my research."'''''<br>
* Fleischman JY, Van den Bergh F, Collins NL, Bowers M, Beard DA, Burant CF (2023) Higher mitochondrial oxidative capacity is the primary molecular differentiator in muscle of rats with high and low intrinsic cardiorespiratory fitness. [[Fleischman 2023 Mol Metab|»Bioblast link«]]
[[Zunica Elizabeth]], from the O2k-Network »[[US LA Baton Rouge Noland RC]]«<br>
:from the O2k-Network »[[US MI Ann Arbor Beard DA]]«
- »'''[[O2k-Feedback]]'''«<br>
|}
|}
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*'''Dec- 15 Fr'''
* '''Mar-29 Fr'''
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'''<big>Next Week</big>'''
'''<big>Next Week</big>'''
  2023 Week 51
  2024 Week 14


*'''Dec- 18 Mo'''
* '''Apr-01 Mo''' April - Parkinson's Disease Awareness Month
:::: '''»[[SBC 2023 Goa IN]]«''' Dec 18-20


:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|''' Why add azide after ascorbate and TMPD when measuring Complex IV activity? '''<br>
|'''"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."''' <br>
The O<sub><small>2</small></sub> flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O<sub><small>2</small></sub> flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement. <br>  
* Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. [[Gispert 2009 PLoS One|»Bioblast link«]]
- »'''[[Complex IV]]'''«, »'''[[Chemical background]] '''«  <br>
:from the O2k-Network »[[US CA San Francisco Nussbaum RL]]« and »[[DE Frankfurt Eckert GP]]«
- communicated by »[[Grings Mateus]]« and »[[Timon-Gomez Alba]]«  
|}
|}
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* '''Apr-02 Tu'''
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*'''Dec- 19 Tu'''
* '''Apr-03 We'''  
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*'''Dec- 20 We'''
* '''Apr-04 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."''' <br>
|''' "Why is it important to check the H<sub>2</sub>O<sub>2</sub> sensitivity when conducting an Amplex UltraRed (AmR) assay?
* Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. [[Noone 2023 Cell Signal|»Bioblast link«]]
The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H<sub>2</sub>O<sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br>
:from the O2k-Network »[[US FL Orlando Goodpaster BH]]« and »[[IE Dublin O Gorman D]]«
 
- »'''[[MiPNet24.10 H2O2 flux analysis]]'''«, »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Amplex UltraRed]'''«  <br>
- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
|}
|}
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*'''Dec- 21 Th'''
* '''Apr-05 Fr'''
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*'''Dec- 22 Fr'''
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Revision as of 12:33, 29 March 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 13
  • Mar-25 Mo
MitoPedia
Living cells or intact cells?

Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.
- »Living cells«, »Gnaiger 2020 BEC Mitochondrial physiology«, »Gnaiger 2020 BEC MitoPathways« 
- communicated by »Leo Elettra« and »Timon-Gomez Alba« 


  • Mar-26 Tu

  • Mar-27 We

  • Mar-28 Th
O2k-Feedback "Introduction to the new models available with the NextGen was immensely helpful. This workshop has been critical for implementation of new techniques and advancement of my research."

Zunica Elizabeth, from the O2k-Network »US LA Baton Rouge Noland RC«
- »O2k-Feedback«


  • Mar-29 Fr

Next Week

2024 Week 14
  • Apr-01 Mo April - Parkinson's Disease Awareness Month
O2k-Publications in the MiPMap "Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."
  • Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. »Bioblast link«
from the O2k-Network »US CA San Francisco Nussbaum RL« and »DE Frankfurt Eckert GP«

  • Apr-02 Tu

  • Apr-03 We

  • Apr-04 Th
O2k-Open SupportInfo: improve "Why is it important to check the H2O2 sensitivity when conducting an Amplex UltraRed (AmR) assay?

The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H2O2 calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.

- »MiPNet24.10 H2O2 flux analysis«, »Amplex UltraRed« 
- communicated by »Grings Mateus« and »Schmitt Sabine«


  • Apr-05 Fr