Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 12:33, 29 March 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 13

Mar-25 Mo

Living cells or intact cells?

Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.
- »Living cells«, »Gnaiger 2020 BEC Mitochondrial physiology«, »Gnaiger 2020 BEC MitoPathways«
- communicated by »Leo Elettra« and »Timon-Gomez Alba«

Mar-26 Tu

Mar-27 We

Mar-28 Th

"Introduction to the new models available with the NextGen was immensely helpful. This workshop has been critical for implementation of new techniques and advancement of my research."

Zunica Elizabeth, from the O2k-Network »US LA Baton Rouge Noland RC«
- »O2k-Feedback«

Mar-29 Fr

Next Week

2024 Week 14

Apr-01 Mo April - Parkinson's Disease Awareness Month

"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."

Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. »Bioblast link«

from the O2k-Network »US CA San Francisco Nussbaum RL« and »DE Frankfurt Eckert GP«

Apr-02 Tu

Apr-03 We

Apr-04 Th

"Why is it important to check the H₂O₂ sensitivity when conducting an Amplex UltraRed (AmR) assay?

The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H₂O₂ calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.

- »MiPNet24.10 H2O2 flux analysis«, »Amplex UltraRed«
- communicated by »Grings Mateus« and »Schmitt Sabine«

Apr-05 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 51
-*'''Dec- 18 Mo'''
+Week 13
-:::: '''»[[SBC 2023 Goa IN]]«''' Dec 18-20
+* '''Mar-25 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Why add azide after ascorbate and TMPD when measuring Complex IV activity? '''<br>
+|''' Living cells or intact cells? '''<br>
-The O<sub><small>2</small></sub> flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O<sub><small>2</small></sub> flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement. <br>
+Living cells are frequently labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.    <br>
-- »'''[[Complex IV]]'''«, »'''[[Chemical background]] '''«  <br>
+- »'''[[Living cells]]'''«, »'''[[BEC 2020.1|Gnaiger 2020 BEC Mitochondrial physiology]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''« <br>
-- communicated by »[[Grings Mateus]]« and »[[Timon-Gomez Alba]]«
+- communicated by »[[Leo Elettra]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Dec- 19 Tu'''
+* '''Mar-26 Tu'''
 ----
-*'''Dec- 20 We'''
+* '''Mar-27 We'''
+----
+* '''Mar-28 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
-|'''"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."''' <br>
+|''' ''"Introduction to the new models available with the NextGen was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my research."'''''<br>
-* Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. [[Noone 2023 Cell Signal|»Bioblast link«]]
+[[Zunica Elizabeth]], from the O2k-Network »[[US LA Baton Rouge Noland RC]]«<br>
-:from the O2k-Network »[[US FL Orlando Goodpaster BH]]« and »[[IE Dublin O Gorman D]]«
+- »'''[[O2k-Feedback]]'''«<br>
 |}
 ----
-*'''Dec- 21 Th'''
+* '''Mar-29 Fr'''
-----
-*'''Dec- 22 Fr'''
 ----
+'''<big>Next Week</big>'''
+Week 14
-'''<big>Next Week</big>'''
+* '''Apr-01 Mo'''  April - Parkinson's Disease Awareness Month
-Week 52
-*'''Dec- 25 Mo'''
+:{|
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+|'''"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."''' <br>
+* Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. [[Gispert 2009 PLoS One|»Bioblast link«]]
+:from the O2k-Network »[[US CA San Francisco Nussbaum RL]]« and »[[DE Frankfurt Eckert GP]]«
+|}
 ----
-*'''Dec- 26 Tu'''
+* '''Apr-02 Tu'''
 ----
-*'''Dec- 27 We'''
+* '''Apr-03 We'''
+----
+* '''Apr-04 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Do you observe a high signal at zero oxygen?'''<br>
+|''' "Why is it important to check the H<sub>2</sub>O<sub>2</sub> sensitivity when conducting an Amplex UltraRed (AmR) assay?
-If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.
+The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H<sub>2</sub>O<sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br>
-<br>
-- »'''[https://wiki.oroboros.at/index.php/High_signal_at_zero_oxygen Leak current test]'''«<br>
+- »'''[[MiPNet24.10 H2O2 flux analysis]]'''«, »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Amplex UltraRed]'''«  <br>
-- communicated by »[[Baglivo Eleonora]]« and »[[Grings Mateus]]«
+- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
 |}
 ----
-*'''Dec- 28 Th'''
+* '''Apr-05 Fr'''
-----
-*'''Dec- 29 Fr'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"Leptin receptor signaling in Schwann cells adjusts catabolic processes, including mitochondrial respiration and autophagy, facilitating nerve repair."''' <br>
-* Sundaram VK, Schütza V, Schröter NH, Backhaus A, Bilsing A, Joneck L, Seelbach A, Mutschler C, Gomez-Sanchez JA, Schäffner E, Sánchez EE, Akkermann D, Paul C, Schwagarus N, Müller S, Odle A, Childs G, Ewers D, Kungl T, Sitte M, Salinas G, Sereda MW, Nave KA, Schwab MH, Ost M, Arthur-Farraj P, Stassart RM, Fledrich R (2023) Adipo-glial signaling mediates metabolic adaptation in peripheral nerve regeneration. [[Sundaram_2023_Cell_Metab|»Bioblast link«]]
-:from the O2k-Network »[[DE_Leipzip_Ost_M]]«
-|}
 ----