Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 12:33, 29 March 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 13

Mar-25 Mo

Living cells or intact cells?

Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.
- »Living cells«, »Gnaiger 2020 BEC Mitochondrial physiology«, »Gnaiger 2020 BEC MitoPathways«
- communicated by »Leo Elettra« and »Timon-Gomez Alba«

Mar-26 Tu

Mar-27 We

Mar-28 Th

"Introduction to the new models available with the NextGen was immensely helpful. This workshop has been critical for implementation of new techniques and advancement of my research."

Zunica Elizabeth, from the O2k-Network »US LA Baton Rouge Noland RC«
- »O2k-Feedback«

Mar-29 Fr

Next Week

2024 Week 14

Apr-01 Mo April - Parkinson's Disease Awareness Month

"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."

Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. »Bioblast link«

from the O2k-Network »US CA San Francisco Nussbaum RL« and »DE Frankfurt Eckert GP«

Apr-02 Tu

Apr-03 We

Apr-04 Th

"Why is it important to check the H₂O₂ sensitivity when conducting an Amplex UltraRed (AmR) assay?

The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H₂O₂ calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.

- »MiPNet24.10 H2O2 flux analysis«, »Amplex UltraRed«
- communicated by »Grings Mateus« and »Schmitt Sabine«

Apr-05 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 01
-* '''Jan-01 Mo'''
+Week 13
-----
-* '''Jan-02Tu'''
+* '''Mar-25 Mo'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' New year, new state? What is REN state? '''<br>
+|''' Living cells or intact cells? '''<br>
-REN state is now distinguished from ROX state: REN is measured in mitochondrial preparations without addition of substrates, whereas ROX is due to oxidative side reactions remaining after inhibition of the electron transfer pathway. <br>
+Living cells are frequently labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.    <br>
-- »'''[[Residual endogenous substrates|REN]]'''«, »'''[[Residual oxygen consumption|ROX]]'''«   <br>
+- »'''[[Living cells]]'''«, »'''[[BEC 2020.1|Gnaiger 2020 BEC Mitochondrial physiology]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''« <br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
+- communicated by »[[Leo Elettra]]« and »[[Timon-Gomez Alba]]«
 |}
+----
+* '''Mar-26 Tu'''
 ----
-* '''Jan-03 We'''
+* '''Mar-27 We'''
 ----
-* '''Jan-04 Th'''
+* '''Mar-28 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
-|'''"Oxygen Consumption Rate (OCR) measurements of fresh and cryopreserved placental specimens are comparable whereas snap frozen procedure impairs mitochondrial activity"''' <br>
+|''' ''"Introduction to the new models available with the NextGen was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my research."'''''<br>
-* Giovarelli M, Serati A, Zecchini S, Guelfi F, Clementi E, Mandò C (2023) Cryopreserved placental biopsies maintain mitochondrial activity for high-resolution respirometry.  [[Giovarelli 2023 Mol Med|»Bioblast link«]]
+[[Zunica Elizabeth]], from the O2k-Network »[[US LA Baton Rouge Noland RC]]«<br>
-:from the O2k-Network »[[IT Milan Clementi E]]«
+- »'''[[O2k-Feedback]]'''«<br>
 |}
 ----
-* '''Jan-05 Fr'''
+* '''Mar-29 Fr'''
------
+----
 '''<big>Next Week</big>'''
-Week 02
+Week 14
+* '''Apr-01 Mo'''  April - Parkinson's Disease Awareness Month
-* '''Jan-08 Mo'''
+:{|
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+|'''"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."''' <br>
+* Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. [[Gispert 2009 PLoS One|»Bioblast link«]]
+:from the O2k-Network »[[US CA San Francisco Nussbaum RL]]« and »[[DE Frankfurt Eckert GP]]«
+|}
+----
+* '''Apr-02 Tu'''
+----
+* '''Apr-03 We'''
+----
+* '''Apr-04 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Do you need to export DatLab traces for your publication? '''<br>
+|''' "Why is it important to check the H<sub>2</sub>O<sub>2</sub> sensitivity when conducting an Amplex UltraRed (AmR) assay?
-Save your graph as an editable picture using "Copy to clipboard" in DatLab 7.4 (.wmf) or "Save" in DatLab 8 (.svg).
+The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H<sub>2</sub>O<sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br>
-<br>
-- »'''[https://wiki.oroboros.at/index.php/Copy_to_clipboard Copy to clipboard in DatLab 7]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ Download DatLab 8]'''«<br>
+- »'''[[MiPNet24.10 H2O2 flux analysis]]'''«, »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Amplex UltraRed]'''«  <br>
-- communicated by »[[Baglivo Eleonora]]« and »[[Grings Mateus]]«
+- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
 |}
 ----
-* '''Jan-09 Tu'''
+* '''Apr-05 Fr'''
-----
-* '''Jan-10 We'''
-:::: '''»[[AICBC 2024 Navi Mumbai IN]]«''' Jan 10-12
-----
-* '''Jan-11 Th'''
 ----
-* '''Jan-12 Fr'''