Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 12:33, 29 March 2024

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-» A future for Ukraine is the future of its science

2024 Week 13

Mar-25 Mo

Living cells or intact cells?

Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.
- »Living cells«, »Gnaiger 2020 BEC Mitochondrial physiology«, »Gnaiger 2020 BEC MitoPathways«
- communicated by »Leo Elettra« and »Timon-Gomez Alba«

Mar-26 Tu

Mar-27 We

Mar-28 Th

"Introduction to the new models available with the NextGen was immensely helpful. This workshop has been critical for implementation of new techniques and advancement of my research."

Zunica Elizabeth, from the O2k-Network »US LA Baton Rouge Noland RC«
- »O2k-Feedback«

Mar-29 Fr

Next Week

2024 Week 14

Apr-01 Mo April - Parkinson's Disease Awareness Month

"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."

Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. »Bioblast link«

from the O2k-Network »US CA San Francisco Nussbaum RL« and »DE Frankfurt Eckert GP«

Apr-02 Tu

Apr-03 We

Apr-04 Th

"Why is it important to check the H₂O₂ sensitivity when conducting an Amplex UltraRed (AmR) assay?

The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H₂O₂ calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.

- »MiPNet24.10 H2O2 flux analysis«, »Amplex UltraRed«
- communicated by »Grings Mateus« and »Schmitt Sabine«

Apr-05 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 05
-* '''Jan-29 Mo'''
+Week 13
-:::: '''»[[E-mit Winter School 2024 Brixen IT]]«''' Jan 28 - Feb 02
-----
+* '''Mar-25 Mo'''
-* '''Jan-30 Tu'''
-:::: '''16:30  [[Gnaiger Erich|Erich Gnaiger]] presenting at [[E-mit Winter School 2024 Brixen IT]]''' - ''Coupling control and pathway control in mitochondrial high-resolution respirometry''
-----
-* '''Jan-31 We'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Can the S-pathway O<sub><small>2</small></sub> flux be derived by subtracting N-pathway O<sub><small>2</small></sub> flux from NS-pathway O<sub><small>2</small></sub> flux?'''<br>
+|''' Living cells or intact cells? '''<br>
-No, the sum of the O<sub><small>2</small></sub> fluxes measured in the presence of NADH-linked substrates alone and succinate as the single substrate is typically higher than using a combination of all these substrates. This effect is described as incomplete additivity: <br>
+Living cells are frequently labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.    <br>
-- »'''[[Additive effect of convergent electron flow]]'''« <br>
+- »'''[[Living cells]]'''«, »'''[[BEC 2020.1|Gnaiger 2020 BEC Mitochondrial physiology]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''« <br>
-- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
+- communicated by »[[Leo Elettra]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Feb-01 Th'''
+* '''Mar-26 Tu'''
+----
+* '''Mar-27 We'''
+----
+* '''Mar-28 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
-|''' ''“Doing the entire hands-on module from start to finish was the best for really “cementing” theory and hands-on application together.”'''''<br>
+|''' ''"Introduction to the new models available with the NextGen was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my research."'''''<br>
-[[Cosgrove Richard]] from Eli Lilly Research Labs, on what he liked best on [[IOC162]]<br>
+[[Zunica Elizabeth]], from the O2k-Network »[[US LA Baton Rouge Noland RC]]«<br>
 - »'''[[O2k-Feedback]]'''«<br>
 |}
 ----
-* '''Feb-02 Fr'''
+* '''Mar-29 Fr'''
 ----
+'''<big>Next Week</big>'''
+Week 14
-'''<big>Next Week</big>'''
+* '''Apr-01 Mo'''  April - Parkinson's Disease Awareness Month
-Week 06
-* '''Feb-05 Mo'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"We demonstrated deficient platelet mitochondrial respiratory chain functions, oxidative phosphorylation (OXPHOS), and electron transfer (ET) capacity with complex I (CI)-linked substrates, and reduced [...] endogenous platelet coenzyme Q10 (CoQ10) concentration in UC [urothelial carcinoma] patients."''' <br>
+|'''"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."''' <br>
-* Palacka P, Gvozdjáková A, Rausová Z, Kucharská J, Slopovský J, Obertová J, Furka D, Furka S, Singh KK, Sumbalová Z (2021) Platelet mitochondrial bioenergetics reprogramming in patients with urothelial carcinoma. [[Palacka 2021 Int J Mol Sci|»Bioblast link«]]
+* Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. [[Gispert 2009 PLoS One|»Bioblast link«]]
-:from the O2k-Network »[[SK Bratislava Sumbalova Z]]«
+:from the O2k-Network »[[US CA San Francisco Nussbaum RL]]« and »[[DE Frankfurt Eckert GP]]«
 |}
 ----
-* '''Feb-06 Tu'''
+* '''Apr-02 Tu'''
 ----
-* '''Feb-07 We'''
+* '''Apr-03 We'''
 ----
-* '''Feb-08 Th'''
+* '''Apr-04 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Can I use the same stock solutions for all reagents for different O2k-chamber volumes?'''<br>
+|''' "Why is it important to check the H<sub>2</sub>O<sub>2</sub> sensitivity when conducting an Amplex UltraRed (AmR) assay?
-"For some reagents, the concentration of the stock solution differs between the 2.0-mL and the 0.5-mL chamber (O2k-sV-Module) to facilitate a precise volume titration."<br>
+The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H<sub>2</sub>O<sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br>
--»'''[[MiPNet09.12 O2k-Titrations]]'''«<br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
+- »'''[[MiPNet24.10 H2O2 flux analysis]]'''«, »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Amplex UltraRed]'''«  <br>
+- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
 |}
 ----
-* '''Feb-09 Fr'''
+* '''Apr-05 Fr'''
 ----