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Difference between revisions of "Template:Current week ecosystem agenda"

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
2024 Week 06


* '''Feb-05 Mo'''
2024 Week 13
 
* '''Mar-25 Mo'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''"We demonstrated deficient platelet mitochondrial respiratory chain functions, oxidative phosphorylation (OXPHOS), and electron transfer (ET) capacity with complex I (CI)-linked substrates, and reduced [...] endogenous platelet coenzyme Q10 (CoQ10) concentration in UC [urothelial carcinoma] patients."''' <br>
|''' Living cells or intact cells? '''<br>
* Palacka P, Gvozdjáková A, Rausová Z, Kucharská J, Slopovský J, Obertová J, Furka D, Furka S, Singh KK, Sumbalová Z (2021) Platelet mitochondrial bioenergetics reprogramming in patients with urothelial carcinoma. [[Palacka 2021 Int J Mol Sci|»Bioblast link«]]
Living cells are frequently labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.    <br>
:from the O2k-Network »[[SK Bratislava Sumbalova Z]]«
- »'''[[Living cells]]'''«, »'''[[BEC 2020.1|Gnaiger 2020 BEC Mitochondrial physiology]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''« <br>
- communicated by »[[Leo Elettra]]« and »[[Timon-Gomez Alba]]«  
|}
|}
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* '''Feb-06 Tu'''  
* '''Mar-26 Tu'''  
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* '''Feb-07 We'''  
* '''Mar-27 We'''  
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* '''Feb-08 Th'''  
* '''Mar-28 Th'''  
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
|'''Can I use the same stock solutions for all reagents for different O2k-chamber volumes?'''<br>
|''' ''"Introduction to the new models available with the NextGen was immensely helpful. This [[MiPNet28.02_IOC162_Schroecken_AT|workshop]] has been critical for implementation of new techniques and advancement of my research."'''''<br>
For some reagents, the concentration of the stock solution differs between the 2.0-mL and the 0.5-mL chamber (O2k-sV-Module) to facilitate a precise volume titration.<br>
[[Zunica Elizabeth]], from the O2k-Network »[[US LA Baton Rouge Noland RC]]«<br>
-»'''[[MiPNet09.12 O2k-Titrations]]'''«<br>
- »'''[[O2k-Feedback]]'''«<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
|}
|}
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* '''Feb-09 Fr'''  
* '''Mar-29 Fr'''
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'''<big>Next Week</big>'''
2024 Week 14


'''<big>Next Week</big>'''
* '''Apr-01 Mo'''  April - Parkinson's Disease Awareness Month
  2024 Week 07


* '''Feb-12 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|''' What is the O<sub>2</sub> solubility factor of the medium (''F''<sub>M</sub>) and why is it important? '''<br>
|'''"Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."''' <br>
''F''<sub>M</sub> expresses the effect of the salt concentration on oxygen solubility relative to pure water, and varies depending on the temperature and composition of the medium. The correct ''F''<sub>M</sub> needs to be entered in DatLab when performing an air calibration. <br>
* Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. [[Gispert 2009 PLoS One|»Bioblast link«]]
- »'''[[Oxygen solubility factor]]'''« , »'''[[Oxygen_calibration_-_DatLab#Air_calibration_in_DatLab|Air calibration in DatLab]]'''« <br>
:from the O2k-Network »[[US CA San Francisco Nussbaum RL]]« and »[[DE Frankfurt Eckert GP]]«
- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«  
|}
|}
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* '''Feb-13 Tu'''  
* '''Apr-02 Tu'''  
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* '''Feb-14 We'''  
* '''Apr-03 We'''  
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* '''Feb-15 Th''' - International Childhood Cancer Day
* '''Apr-04 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''"Deleterious mtDNA mutations contribute significantly to the tumorigenesis of pediatric cancers"''' <br>
|''' "Why is it important to check the H<sub>2</sub>O<sub>2</sub> sensitivity when conducting an Amplex UltraRed (AmR) assay?
* Triska P, Kaneva K, Merkurjev D, Sohail N, Falk MJ, Triche TJ Jr, Biegel JA, Gai X (2019) Landscape of germline and somatic mitochondrial DNA mutations in pediatric malignancies. [[Triska 2019 Cancer Res|»Bioblast link«]]
The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H<sub>2</sub>O<sub>2</sub> calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br>
:from the O2k-Network »[[US_PA_Philadelphia_Falk_MJ]]«
 
- »'''[[MiPNet24.10 H2O2 flux analysis]]'''«, »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Amplex UltraRed]'''«  <br>
- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
|}
|}
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* '''Feb-16 Fr'''  
* '''Apr-05 Fr'''
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Revision as of 12:33, 29 March 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 13
  • Mar-25 Mo
MitoPedia
Living cells or intact cells?

Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.
- »Living cells«, »Gnaiger 2020 BEC Mitochondrial physiology«, »Gnaiger 2020 BEC MitoPathways« 
- communicated by »Leo Elettra« and »Timon-Gomez Alba« 


  • Mar-26 Tu

  • Mar-27 We

  • Mar-28 Th
O2k-Feedback "Introduction to the new models available with the NextGen was immensely helpful. This workshop has been critical for implementation of new techniques and advancement of my research."

Zunica Elizabeth, from the O2k-Network »US LA Baton Rouge Noland RC«
- »O2k-Feedback«


  • Mar-29 Fr

Next Week

2024 Week 14
  • Apr-01 Mo April - Parkinson's Disease Awareness Month
O2k-Publications in the MiPMap "Although the chain of events leading to this respiration deficit is currently not understood, the findings constitute a robust assay for the progressive pathology induced by PINK1 deficiency."
  • Gispert S, Ricciardi F, Kurz A, Azizov M, Hoepken HH, Becker D, Voos W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Barrera M, Reichert AS, Rüb U, Chen A, Nussbaum RL, Auburger G (2009) Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. »Bioblast link«
from the O2k-Network »US CA San Francisco Nussbaum RL« and »DE Frankfurt Eckert GP«

  • Apr-02 Tu

  • Apr-03 We

  • Apr-04 Th
O2k-Open SupportInfo: improve "Why is it important to check the H2O2 sensitivity when conducting an Amplex UltraRed (AmR) assay?

The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H2O2 calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.

- »MiPNet24.10 H2O2 flux analysis«, »Amplex UltraRed« 
- communicated by »Grings Mateus« and »Schmitt Sabine«


  • Apr-05 Fr