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Latest revision as of 08:23, 15 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

Azzi A, Gnaiger E (2024) Tributes to pioneers in bioenergetics - wandering in the garden of memories. Bioenerg Commun 2024.3.v1. https://doi.org/10.26124/bec:2024-0003

- »Bioblast link«

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

Next Week

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

@@ Line 1: / Line 1: @@
+{{Template:Science for Ukraine}}
-Week 27
+Week 20
-* '''Jun-29 Mo'''
+* '''May-13 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable)<br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- » [[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«
+<br>
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
+|}
+----
+* '''May-14 Tu'''
+----
+* '''May-15 We'''
+:{|
+| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
+|Azzi A, Gnaiger E (2024) Tributes to pioneers in bioenergetics - wandering in the garden of memories. Bioenerg Commun 2024.3.v1. https://doi.org/10.26124/bec:2024-0003<br>
+- [[Azzi_2024_BEC-Tributes |»Bioblast link«]]
 |}
 ----
-* '''Jun-30 Tu'''
+* '''May-16 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]«
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
-*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|»Bioblast link«]]
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Jul-01 We'''
+* '''May-17 Fr'''
+----
+'''<big>Next Week</big>'''
+Week 21
+* '''May-20 Mo'''
+:{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
+----
+* '''May-21 Tu'''
 ----
-* '''Jul-02 Th'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
- | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
- | '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-- loss of oxidative capacity during the time course of the respirometric assay,<br>
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-- insufficient [[Uncoupler|uncoupler]] concentrations,<br>
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
-- over titration of an uncoupler, which inhibits ET capacity,<br>
-- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.
-Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]«
 |}
 ----
-* '''Jul-03 Fr'''
+* '''May-23 Th'''
-:{|
+----
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
+* '''May-24 Fr'''
-|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br>
+----
-: from the O2k-Network »[[US CO Fort Collins Chicco AJ]]« <br>
-*           Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|»Bioblast link«]]
-|}