Difference between revisions of "Template:Current week ecosystem agenda"

Latest revision as of 08:23, 15 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

Azzi A, Gnaiger E (2024) Tributes to pioneers in bioenergetics - wandering in the garden of memories. Bioenerg Commun 2024.3.v1. https://doi.org/10.26124/bec:2024-0003

- »Bioblast link«

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

Next Week

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

@@ Line 1: / Line 1: @@
-Week 42
+{{Template:Science for Ukraine}}
-* '''Oct-12 Mo''' [http://www.metabesity2020.com/ Targeting Metabesity 2020 Virtual Event] Oct 12-15
+Week 20
+* '''May-13 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''If using the [[Amplex UltraRed]] assay, when should the background H<sub>2</sub>O<sub>2</sub> calibration be performed?
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-Background H<sub>2</sub>O<sub>2</sub> calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H<sub>2</sub>O<sub>2</sub> is prepared, or if a different respiration medium is used. As the H<sub>2</sub>O<sub>2</sub> stock solution needs to be freshly prepared, it is recommended to perform a background H<sub>2</sub>O<sub>2</sub> calibration before each experiment and at a minimum once per experimental day. <br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- »[[Instrumental:_Browse_DL-Protocols_and_templates|How to find the background H<sub>2</sub>O<sub>2</sub> calibration protocol in DatLab]] « select  “AmR calibration”  for the background H<sub>2</sub>O<sub>2</sub> calibration protocol <br>
+<br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 |}
 ----
-* '''Oct-13 Tu'''
+* '''May-14 Tu'''
-:::: [[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Virtual Event]]'''</big>«
 ----
-* '''Oct-14 We'''
+* '''May-15 We'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
-|'''Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction? '''<br>
+|Azzi A, Gnaiger E (2024) Tributes to pioneers in bioenergetics - wandering in the garden of memories. Bioenerg Commun 2024.3.v1. https://doi.org/10.26124/bec:2024-0003<br>
-:from the O2k-Network »[[US VA Richmond Lesnefsky EJ]]« <br>
+- [[Azzi_2024_BEC-Tributes |»Bioblast link«]]
-*Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. [[Mohsin 2020 Arch Biochem Biophys|»Bioblast link«]]
 |}
 ----
-* '''Oct-15 Th'''
+* '''May-16 Th'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' How can you reduce the amount of biological sample needed for high-resolution respirometry?  '''<br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-The O2k-sV-Module  provides all the components necessary for a smaller operation volume (0.5 mL) whereby the sample amount used for measuring oxygen flux can be reduced.   <br>
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
-- »'''[[O2k-sV-Module]]'''«  <br>
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
 |}
 ----
-* '''Oct-16 Fr'''
+* '''May-17 Fr'''
+----
+'''<big>Next Week</big>'''
+Week 21
+* '''May-20 Mo'''
 :{|
-| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-| '''Which SUIT protocol can you use to evaluate mitochondrial respiration of [[PBMC]] and [[Platelet|platelets]]?'''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-The following SUIT protocols will guide you through the titration experiments for these cell models <br>
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-- »'''[[SUIT-001 O2 ce-pce D004]]'''« and »'''[[SUIT-002 O2 ce-pce D007a]]'''« <br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- communicated by »[[Cecatto Cristiane]]« and »[[Garcia-Souza Luiz F]]«
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
+|}
+----
+* '''May-21 Tu'''
+----
+* '''May-22 We''' - International Day for Biological Diversity
+:{|
+| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-* '''Oct-17 Sa'''
+* '''May-23 Th'''
+----
+* '''May-24 Fr'''
 ----
-* '''Oct-18 Su'''
-:::: »<big>'''[[ISCaM 2020 Virtual Event]]'''</big>«