Difference between revisions of "Template:Current week ecosystem agenda"

Latest revision as of 12:04, 29 April 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

May-10 Fr

@@ Line 1: / Line 1: @@
-Week 43
+{{Template:Science for Ukraine}}
-* '''Oct-19 Mo'''
+Week 18
+* '''Apr-29 Mo'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?'''<br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.<br>
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
--  You can read more about it in the following publication: »[[Krumschnabel 2014 Methods Enzymol]]« <br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Oct-20 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]]
+* '''Apr-30 Tu'''
+----
+* '''May-01 We'''
 ----
-* '''Oct-21 We'''
+* '''May-02 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Blood cell respirometry might be an attractive alternative to skeletal- and cardiac muscle-based assessment of mitochondrial function in human studies '''<br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-:from the O2k-Network »[[US NC Winston-Salem Molina AJA]]«
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-*Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77.[[Tyrrell 2016 Redox Biol|»Bioblast link«]]
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
+----
+* '''May-03 Fr'''
-----
+'''<big>Next Week</big>'''
-* '''Oct-22 Th'''
+Week 19
+* '''May-06 Mo'''
 :{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-| width="110" align="center" | [[File:BEC-logo.png|left|100px|link=Bioenergetics Communications|Bioenergetics Communications]]
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-|''' LEAK respiration relates to intrinsic uncoupling.   '''<br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.  <br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC2020.1: Mitochondrial physiology]'''« - Table 2. Terms on respiratory coupling and uncoupling  <br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]« and »[[Gnaiger Erich]]«
 |}
 ----
-* '''Oct-23 Fr'''
+* '''May-07 Tu'''
-:{|
+----
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
+* '''May-08 We'''
-|'''Simultaneous measurement of O<sub>2</sub> flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system''' <br>
+----
-: from the O2k-Network »[[AT Innsbruck Oroboros]]« and »[[AT Innsbruck Gnaiger E]]«<br>
+* '''May-09 Th'''
-* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.   [[Krumschnabel 2014 Methods Enzymol|»Bioblast link«]]
+----
-|}
+* '''May-10 Fr'''