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Latest revision as of 09:37, 21 May 2024

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-» A future for Ukraine is the future of its science

2024 Week 21

May-20 Mo

How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test«
- communicated by »Baglivo Eleonora« and »Schmitt Sabine«

May-21 Tu

May-22 We - International Day for Biological Diversity

"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."

Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«

from the O2k-Network »NO Tromsoe Wood SH«

May-23 Th

May-24 Fr

Next Week

2024 Week 22

May-27 Mo

Same experimental conditions, but different O₂ fluxes in the left and right chambers?

Check for instrumental and operational factors that could be causing O₂ flux differences between the O2k-chambers.
- »Different O2 fluxes in left and right chamber«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-28 Tu

May-29 We

May-30 Th

"Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"

- »MiPNet20.06 Isolation mouse heart mitochondria«
- communicated by »Cardoso Luiza« and »Leo Elettra«

May-31 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 42
+Week 21
-*'''Oct- 16 Mo'''
+* '''May-20 Mo'''
-:::: '''»[[2nd Workshop on Mitochondrial Functional Diagnostics Innsbruck AT]]«'''
-----
-*'''Oct- 17 Tu'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''Noticing higher values of ''J''°1 when using the O2k sV-chamber (0.5 mL) compared to the O2k 2mL-chamber? '''<br>
+|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
-The instrumental oxygen background flux, ''J''°O<sub>2</sub> is volume-dependent in the closed chamber; therefore, the values of ''J''°1 are about four times higher in the sV chamber.
+Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
-<br>
+- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
-- »'''[[O2k-sV-Module|O2k-sV-Module]]'''«, »'''[[MiPNet06.03 POS-calibration-SOP|POS calibration-SOP]]'''«<br>
+- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
+|}
-|}
 ----
-*'''Oct- 18 We'''
+* '''May-21 Tu'''
 ----
-*'''Oct- 19 Th'''
+* '''May-22 We''' - International Day for Biological Diversity
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"MDH2 is a metabolic switch rewiring [electron transfer system] and TCA fuelling"''' <br>
+|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
-* Molinié T, Cougouilles E, David C, Cahoreau E, Portais JC, Mourier A (2022) MDH2 produced OAA is a metabolic switch rewiring the fuelling of respiratory chain and TCA cycle. [[Molinie 2022 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
+* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
-:from the O2k-Network »[[FR Bordeaux Mourier A]]«
+:from the O2k-Network »[[NO Tromsoe Wood SH]]«
 |}
 ----
-*'''Oct- 20 Fr'''
+* '''May-23 Th'''
 ----
+* '''May-24 Fr'''
+----
 '''<big>Next Week</big>'''
-Week 43
+Week 22
-*'''Oct- 23 Mo'''
+* '''May-27 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|''' ''“Thanks for this excellent guidance and support thus far.”'''''<br>
+|'''Same experimental conditions, but different O<sub>2</sub> fluxes in the left and right chambers?'''<br>
-[[Maarman Gerald J|Maarman Gerald J]], from the O2k-Network Lab »[[ZA Cape Town Maarman GJ]]«<br>
+Check for instrumental and operational factors that could be causing O<sub>2</sub> flux differences between the O2k-chambers.
-- »'''[[O2k-Feedback]]'''«<br>
+<br>
+- »'''[[Different O2 fluxes in left and right chamber]]'''«<br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-*'''Oct- 24 Tu'''
+* '''May-28 Tu'''
+----
+* '''May-29 We'''
+----
+* '''May-30 Th'''
 :{|
-| width="100" align="center" |[[File:MitoFit Preprints.png|100px|link=https://wiki.oroboros.at/images/e/eb/Garcia_Menendez_2023_MitoFit.pdf |MitoFit pdf]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''A theoretical communication from Garcia Menendez on electromagnetic coupling hypothesis.'''<br>
+|''' "Harmonization of isolation protocols yielding intact and enzymatically active mitochondria are critical to assess mitochondrial respiratory function"'''<br>
-* Garcia Menendez R (2023) Update and a new statement of the electromagnetic coupling hypothesis to explain the translocation of protons in mitochondria, bacteria and chloroplasts. MitoFit Preprints 2023.8. https://doi.org/10.26124/mitofit:2023-0008
+- »'''[[MiPNet20.06_IsolationMouseHeart-mt|MiPNet20.06 Isolation mouse heart mitochondria]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Leo Elettra]]«
 |}
 ----
-*'''Oct- 25 We'''
+* '''May-31 Fr'''
-----
-*'''Oct- 26 Th'''
-----
-*'''Oct- 27 Fr'''
 ----