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Difference between revisions of "Template:Current week ecosystem agenda"

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
2023 Week 51


*'''Dec- 18 Mo'''
2024 Week 20
:::: '''»[[SBC 2023 Goa IN]]«''' Dec 18-20


* '''May-13 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
|''' Why add azide after ascorbate and TMPD when measuring Complex IV activity? '''<br>
|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
The O<sub><small>2</small></sub> flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O<sub><small>2</small></sub> flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement. <br>  
Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.  
- »'''[[Complex IV]]'''«, »'''[[Chemical background]] '''«  <br>
<br>
- communicated by »[[Grings Mateus]]« and »[[Timon-Gomez Alba]]«  
- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
|}
|}
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*'''Dec- 19 Tu'''
* '''May-14 Tu'''  
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*'''Dec- 20 We'''
* '''May-15 We'''
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* '''May-16 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."''' <br>
|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
* Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. [[Noone 2023 Cell Signal|»Bioblast link«]]
- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
:from the O2k-Network »[[US FL Orlando Goodpaster BH]]« and »[[IE Dublin O Gorman D]]«
- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
|}
|}
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*'''Dec- 21 Th'''
* '''May-17 Fr'''
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*'''Dec- 22 Fr'''
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'''<big>Next Week</big>'''


'''<big>Next Week</big>'''
  2024 Week 21
  2023 Week 52


*'''Dec- 25 Mo'''
* '''May-20 Mo'''  
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|''' How do I determine the optimal oligomycin concentration for my assay?'''<br>
Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity. <br>
- »'''[https://wiki.oroboros.at/index.php/Oligomycin#Omy_titration_test Oligomycin titration test]'''« <br>
- communicated by »[[Baglivo Eleonora]]« and »[[Schmitt Sabine]]«
|} 
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*'''Dec- 26 Tu'''
* '''May-21 Tu'''  
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*'''Dec- 27 We'''
* '''May-22 We''' - International Day for Biological Diversity
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Do you observe a high signal at zero oxygen?'''<br>
|'''"We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."''' <br>
If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.  
* Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. [[Ciccone 2024 J Exp Biol|»Bioblast link«]]
<br>
:from the O2k-Network »[[NO Tromsoe Wood SH]]«
- »'''[https://wiki.oroboros.at/index.php/High_signal_at_zero_oxygen Leak current test]'''«<br>
- communicated by »[[Baglivo Eleonora]]« and »[[Grings Mateus]]«
|}
|}
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*'''Dec- 28 Th'''
* '''May-23 Th'''  
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*'''Dec- 29 Fr'''
* '''May-24 Fr'''
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''"Leptin receptor signaling in Schwann cells adjusts catabolic processes, including mitochondrial respiration and autophagy, facilitating nerve repair."''' <br>
* Sundaram VK, Schütza V, Schröter NH, Backhaus A, Bilsing A, Joneck L, Seelbach A, Mutschler C, Gomez-Sanchez JA, Schäffner E, Sánchez EE, Akkermann D, Paul C, Schwagarus N, Müller S, Odle A, Childs G, Ewers D, Kungl T, Sitte M, Salinas G, Sereda MW, Nave KA, Schwab MH, Ost M, Arthur-Farraj P, Stassart RM, Fledrich R (2023) Adipo-glial signaling mediates metabolic adaptation in peripheral nerve regeneration. [[Sundaram_2023_Cell_Metab|»Bioblast link«]]
:from the O2k-Network »[[DE_Leipzip_Ost_M]]«
|}
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Latest revision as of 09:04, 13 May 2024

Gentle Science
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A future for Ukraine is the future of its science
2024 Week 20
  • May-13 Mo
O2k-Open SupportInfo: take care Do you see fluctuations of O2 slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O2 slope negative.
- »Fluctuations of O2 slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«


  • May-14 Tu

  • May-15 We

  • May-16 Th
O2k-Open SupportInfo: improve "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«


  • May-17 Fr

Next Week

2024 Week 21
  • May-20 Mo
MitoPedia
How do I determine the optimal oligomycin concentration for my assay?

Perform the oligomycin titration test, and adjust your oligomycin concentration to minimize the inhibition of ET capacity.
- »Oligomycin titration test« 
- communicated by »Baglivo Eleonora« and »Schmitt Sabine« 


  • May-21 Tu

  • May-22 We - International Day for Biological Diversity
O2k-Publications in the MiPMap "We have identified a circadian clock phase-dependent change in Complex I coupling efficiency, demonstrating for the first time a mitochondria–clock interaction in the hooded seal."
  • Ciccone C, Kante F, Folkow LP, Hazlerigg DG, West AC, Wood SH (2024) Circadian coupling of mitochondria in a deep-diving mammal. »Bioblast link«
from the O2k-Network »NO Tromsoe Wood SH«

  • May-23 Th

  • May-24 Fr