Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 09:08, 27 October 2020

2020 Week 44

Oct-26 Mo

"TRANSMIT: THE ROAD TO MEET THE MITOS" – the interactive TRANSMIT booklet for children is available for download in multiple languages

- »https://www.transmit-project.eu/the-transmit-booklet-for-children-is-now-available-online-for-download/

Did you know that oxygen flux can be converted to flux control ratios (FCRs) directly in DatLab?

Use this function to easily plot your experimental traces as FCRs, or to analyze your data.
- »FCR in DatLab«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«

Oct-27 Tu

The mitochondria's role in the immunity of patients with SARS-CoV infections - a review from an O2k-Network lab

from the O2k-Network »CH Lausanne Place N«

Burtscher J, Cappellano G, Omori A, Koshiba T, Millet GP (2020) Mitochondria - in the crossfire of SARS-CoV-2 and immunity. iScience [Epub ahead of print]. »Bioblast link«

Oct-28 We

Flow or flux - what is the difference?

Rate can be expressed both as flow (I) and flux (J). Flow is used if the sample X can be quantified as count (N_X; the number of cells, organisms), and changes proportionally with the size of X. Flux is a size-independent quantity where the rate is expressed per mass or volume of sample.
- »BEC2020.1: Mitochondrial physiology« - Box 2. Metabolic flows and fluxes: vectoral, vectorial, and scalar
- communicated by »Cecatto Cristiane« and »Komlodi Timea« and »Gnaiger Erich«

Oct-29 Th

Would you like to monitor O₂ flux in response to light?

Read about the PB-Module, the new module in the NextGen-O2k that will enable experiments in PhotoBiology!
- »PB-Module«, »PhotoBiology«
- communicated by »Huete-Ortega Maria« and »Schmitt Sabine« and »Tindle-Solomon Lisa«

Oct-30 Fr

Measuring ATP-ADP exchange via adenine nucleotide translocase (ANT) using the fluorophore Magnesium Green (MgG)

from the O2k-Network »HU Budapest Chinopoulos C«

Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«

@@ Line 1: / Line 1: @@
-Week 43
+Week 44
-* '''Oct-19 Mo'''
+* '''Oct-26 Mo'''
 :{|
+| width="110" align="center" | [[File:TRANSMIT Acronym.jpg|left|70px|TRANSMIT|link=TRANSMIT]]
+|      '''"TRANSMIT: THE ROAD TO MEET THE MITOS"''' – the interactive [[TRANSMIT]] booklet for children is available for download in multiple languages  <br>
+- »https://www.transmit-project.eu/the-transmit-booklet-for-children-is-now-available-online-for-download/ <br>
+|}
+:{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?'''<br>
+|''' Did you know that oxygen flux can be converted to ''flux control ratios'' (''FCRs'') directly in DatLab?'''<br>
-Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.<br>
+Use this function to easily plot your experimental traces as ''FCRs'', or to analyze your data.
--  You can read more about it in the following publication: »[[Krumschnabel 2014 Methods Enzymol]]« <br>
+<br>
+- »'''[[Flux_control_ratio#DatLab|''FCR'' in DatLab]]'''« <br>
 - communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
 |}
 ----
-* '''Oct-20 Tu''' - [[12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme|ÖGMBT Life Science Tuesdays]]
+* '''Oct-27 Tu'''
+:{|
+| width="100" align="center" |[[Image:O2k-Network.png|left|70px|O2k-Network|link=O2k-Network]][[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]
+| '''The mitochondria's role in the immunity of patients with SARS-CoV infections - a review from an O2k-Network lab'''
+:from the O2k-Network »[[CH Lausanne Place N]]« <br>
+*Burtscher J, Cappellano G, Omori A, Koshiba T, Millet GP (2020) Mitochondria - in the crossfire of SARS-CoV-2 and immunity. iScience [Epub ahead of print]. [[Burtscher 2020 iScience|»Bioblast link«]]
+|}
 ----
-* '''Oct-21 We'''
+* '''Oct-28 We'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[File:BEC-logo.png|left|100px|link=Bioenergetics Communications|Bioenergetics Communications]]
-|'''Blood cell respirometry might be an attractive alternative to skeletal- and cardiac muscle-based assessment of mitochondrial function in human studies '''<br>
+|''' Flow or flux - what is the difference? '''<br>
-:from the O2k-Network »[[US NC Winston-Salem Molina AJA]]«
+Rate can be expressed both as flow (''I'') and flux (''J''). Flow is used if the sample ''X'' can be quantified as [[count]] (''N<sub>X</sub>''; the number of cells, organisms), and changes proportionally with the size of ''X''. Flux is a size-independent quantity where the rate is expressed per mass or volume of sample.   <br>
-*Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77.[[Tyrrell 2016 Redox Biol|»Bioblast link«]]
+- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC2020.1: Mitochondrial physiology]'''« - Box 2. Metabolic flows and fluxes: vectoral, vectorial, and scalar  <br>
+- communicated by »[[Cecatto Cristiane]]« and »[[Komlodi Timea]]« and »[[Gnaiger Erich]]«
 |}
-* '''Oct-21 We'''
+----
+* '''Oct-29 Th'''
 :{|
 | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Human [[PBMC]] and [[Platelet|Platelets]] - samples for high-resolution respirometry obtained by minimally invasive procedures.'''<br>
+|''' Would you like to monitor O<sub>2</sub> flux in response to light?  '''<br>
-We compared isolation procedures for blood cells.<br>
+Read about the PB-Module, the new module in the NextGen-O2k that will enable experiments in PhotoBiology! <br>
-- »'''[[MiPNet21.17_BloodCellsIsolation|MiPNet21.17 Blood Cells Isolation]]'''«  <br>
+- »'''[[PB-Module]]'''«, »'''[[PhotoBiology]]'''«  <br>
-- communicated by »[[Cecatto Cristiane]]« and »[[Garcia-Souza Luiz F]]«
+- communicated by »[[Huete-Ortega Maria]]« and »[[Schmitt Sabine]]« and »[[Tindle-Solomon Lisa]]«
-|}
-----
-* '''Oct-22 Th'''
-:{|
-| width="110" align="center" | [[File:BEC-logo.png|left|100px|link=Bioenergetics Communications|Bioenergetics Communications]]
-|''' LEAK respiration relates to intrinsic uncoupling.   '''<br>
-Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.  <br>
-- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC2020.1: Mitochondrial physiology]'''« - Table 2. Terms on respiratory coupling and uncoupling  <br>
-- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]« and »[[Gnaiger Erich]]«
 |}
 ----
-* '''Oct-23 Fr'''
+* '''Oct-30 Fr'''
 :{|
 | width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
-|'''Simultaneous measurement of O<sub>2</sub> flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system''' <br>
+|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br>
-: from the O2k-Network »[[AT Innsbruck Oroboros]]« and »[[AT Innsbruck Gnaiger E]]«<br>
+: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br>
-* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.   [[Krumschnabel 2014 Methods Enzymol|»Bioblast link«]]
+* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48.  [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]
 |}