Template:Current week ecosystem agenda
2020 Week 37
- Sep-07 Mo
Why it is necessary to perform multiple H2O2 calibrations when using the Amplex UltraRed (AmR) assay?
The sensitivity to H2O2 of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H2O2 calibration before sample addition, after sample addition and multiple times during the experiment.
- »Komlodi 2018 Methods Mol Biol«
The DLPs for AmR SUIT protocols already come with multiple steps of H2O2 calibrations implemented.
- » SUITbrowser«
- communicated by »Komlodi Timea« and »Cardoso Luiza«
- Sep-08 Tu
Alternative oxidase (AOX) can prevent acute hypoxic pulmonary vasoconstriction in mouse lung
- from the O2k-Network »DE Giessen Weissmann N«, »FI Tampere Dufour E«, »FI Helsinki Jacobs HT« and »DE Jena Szibor M«
- Sommer N, Alebrahimdehkordi N, Pak O, Knoepp F, Strielkov I, Scheibe S, Dufour E, Andjelekovic A, Sydykov A, Saraji A, Petrovic A, Quanz K, Hecker M, Kumar M, Wahl J, Kraut S, Seeger W, Schermuly RT, Ghofrani HA, Ramser K, Braun T, Jacobs HT, Weissmann N, Szibor M (2020) Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Sci Adv 6:eaba0694. »Bioblast link«
- Sep-09 We
- Sep-10 Th
What is the limitation of State 3 respiration compared to OXPHOS capacity?
OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, Pi, fuel substrates and O2. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of excess E-P capacity due to kinetic limitations of the phosphorylation system.
- »BEC: Mitochondrial physiology« - Table 3 and several figures.
- communicated by » Cecatto Cristiane « and » Komlodi Timea «
- Sep-11 Fr
