Difference between revisions of "Template:Next oroboros ecosystem agenda"

Revision as of 08:25, 28 June 2021

2021 Week 27

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Safranin or TPP⁺ - which method to choose for mt-membrane potential (mtMP) detection?

If the measurement of mtMP in absolute mV values is required, then TPP⁺ is the method of choice. However, a linear relationship between fluorescence intensity and mtMP can be used to calculate absolute mtMP values with safranin. Both safranin and TPP⁺ are suitable for quantification of differences.
- »TPP vs safranin«

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»Chlamy 2021 Ile des Embiez FR« Aug 29 - Sep 3

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@@ Line 1: / Line 1: @@
-Week 26
-*'''Jun-28 Mo'''
-:{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' Do I need to calibrate the fluorescence signal with safranin?'''<br>
-It is possible to run the experiment without calibration, presenting only the fluorescence intensity plot. However, a simple two-point or multiple-point calibration for safranin concentration is possible.
-<br>
-- »'''[https://wiki.oroboros.at/index.php/Safranin#Safranin_calibration Safranin calibration]'''«<br>
-|}
-----
-*'''Jun-29 Tu'''
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-*'''Jun-30 We'''
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-*'''Jul-01 Th'''
-:::: '''18:00-19:00 ([https://time.is/GMT+2 GMT+2]): Oroboros Instruments at [[ISAP 2021 Virtual]]''' - ''Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry''
-----
-*'''Jul-02 Fr'''
-:{|
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
-|'''Inflammation - a cause of mitochondrial dysfunction and hepatic cell death in mice fed a high-fat diet''' <br>
-:from the O2k-Network »[[US MO St Louis Gross RW]]«
-* Moon SH, Dilthey BG, Liu X, Guan S, Sims HF, Gross RW (2021) High-Fat diet activates liver iPLA<sub>2</sub>γ generating eicosanoids that mediate metabolic stress. J Lipid Res 62: 100052. [Epub ahead of print].  [[Moon 2021 J Lipid Res|»Bioblast link«]]
-|}
 Week 27