Template:Next oroboros ecosystem agenda

2020 Week 42

Oct-12 Mo Targeting Metabesity 2020 Virtual Event Oct 12-15

If using the Amplex UltraRed assay, when should the background H₂O₂ calibration be performed?

Background H₂O₂ calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H₂O₂ is prepared, or if a different respiration medium is used. As the H₂O₂ stock solution needs to be freshly prepared, it is recommended to perform a background H₂O₂ calibration before each experiment and at a minimum once per experimental day.
- »How to find the background H₂O₂ calibration protocol in DatLab « select “AmR calibration” for the background H₂O₂ calibration protocol
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Oct-13 Tu

»FAT4BRAIN School IOC147 Virtual Event«

Oct-14 We

Calcium overload: The mechanism by which ER stress leads to Complex I dysfunction?

from the O2k-Network »US VA Richmond Lesnefsky EJ«

Mohsin AA, Thompson J, Hu Y, Hollander J, Lesnefsky EJ, Chen Q (2020) Endoplasmic reticulum stress-induced Complex I defect: Central role of calcium overload. Arch Biochem Biophys 683:108299. »Bioblast link«

Oct-15 Th

Oct-16 Fr

Oct-18 Su

»ISCaM 2020 Virtual Event«

2020 Week 43

Oct-19 Mo

Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?

Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.
- You can read more about it in the following publication: »Krumschnabel 2014 Methods Enzymol«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«

Oct-20 Tu - ÖGMBT Life Science Tuesdays

Oct-21 We

Oct-22 Th

Oct-23 Fr

Simultaneous measurement of O2 flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system

from the O2k-Network »AT Innsbruck Oroboros« and »AT Innsbruck Gnaiger E«

Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Bioblast link«