Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Template:SUIT-003

From Bioblast
MitoPedia: SUIT

Steps and respiratory states

Ce1;ce2(Omy);ce3U-.png


Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1

ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.

ce2Omy L ce1;ce2Omy

Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.

  • The addition of oligomycin or its carrier is optional depending on the experimental question to resolve.
ce3U E ce1;ce2Omy;ce3U

Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E.

ce4Rot ROX ce1;ce2Omy;ce3U;ce4Rot

Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.