Difference between revisions of "Template:SUIT-010"

Revision as of 15:32, 28 January 2019

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
ce1	ROUTINE			ce1 ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
ce2Rot	ROX		CII	ce1;ce2Rot Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway.
ce3S	S_L	S	CII	ce1;ce2Rot;ce3S Succinate, S ( type S-pathway to Q). Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
ce4D	S_P	S	CII	ce1;ce2Rot;ce3S;ce4D Succinate, S ( type S-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
1Dig	S_P	S	CII	ce1;ce2Rot;ce3S;ce4D;1Dig Succinate, S ( type S-pathway to Q). Multiple Digitonin titrations for complete plasma membrane permeabilization. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
1c	S_P	S	CII	ce1;ce2Rot;ce3S;ce4D;1Dig;1c Succinate, S ( type S-pathway to Q). Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.

Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Coupling control

» Coupling control state

Pathway control

» Electron transfer pathway

Main fuel substrates

» Glutamate, G

Glossary

» List of SUIT states

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-{{Template:SUIT S}} {{Template:SUIT L(n)}}
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-{{Template:SUIT S}} {{Template:SUIT OXPHOS}}
+* {{Template:SUIT S}}
+* {{Template:SUIT OXPHOS}}
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-{{Template:SUIT S}} {{Template:SUIT Dig*}} {{Template:SUIT OXPHOS}}
+* {{Template:SUIT S}}
+* {{Template:SUIT Dig*}}
+* {{Template:SUIT OXPHOS}}
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-{{Template:SUIT S}} {{Template:SUIT c}} {{Template:SUIT OXPHOS}}
+* {{Template:SUIT S}}
+* {{Template:SUIT c}}
+* {{Template:SUIT OXPHOS}}
 |}
 {{Template:Keywords in SUIT protocols}}